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目的:探讨血管紧张素转化酶基因I/D多态性与青海省贵德地区汉族冠心病(CHD)患者之间的相关关系。方法:冠心病患者89例和健康汉族75例,应用聚合酶链反应(PCR)技术检测两组人群血管紧张素转换酶(ACE)基因插入/缺失(I/D)多态性,观察II、ID、DD基因型频率。结果:通过冠心病组和正常对照组的ACE基因I/D多态性的比较发现,冠心病组DD、ID和II基因型频率分别为33.7%、46.1%、20.2%,D、I等位基因频率分别为56.7%、43.3%;健康对照组分别为20.0%、44.0%、36.0%和42.0%、58.0%。冠心病组血管紧张素转化酶基因型及等位基因频率与对照组相比有显著性差异(P<0.05)。ACE的DD基因型频率和D等位基因频率在冠心病组中显著增加(P<0.05)。结论:贵德地区冠心病与ACE基因多态性有一定相关性,ACE、DD基因型可能是冠心病的危险因子。
Objective: To investigate the relationship between angiotensin I converting enzyme gene I / D polymorphism and Han Chinese patients with coronary heart disease (CHD) in Qingde district of Qinghai Province. Methods: 89 patients with coronary heart disease and 75 healthy Han people were enrolled in this study. ACE gene insertion / deletion (I / D) polymorphism was detected by polymerase chain reaction (PCR) ID, DD genotype frequency. Results: Comparison of ACE gene I / D polymorphism between coronary heart disease group and normal control group showed that the frequencies of DD, ID and II genotypes in CHD group were 33.7%, 46.1%, 20.2%, D, I The gene frequencies were 56.7% and 43.3% respectively. The healthy controls were 20.0%, 44.0%, 36.0% and 42.0%, 58.0% respectively. The genotype and allele frequencies of angiotensin converting enzyme in coronary heart disease group were significantly different from those in control group (P <0.05). The DD genotype frequency and D allele frequency of ACE in coronary heart disease group were significantly increased (P <0.05). Conclusion: There is a correlation between coronary heart disease and ACE gene polymorphism in the Gui De region. ACE and DD genotypes may be risk factors of coronary heart disease.