九连小檗的叶片,在含有2,4-D(1mg/l),KT(0.1mg/l),琼脂(0.8%),蔗糖(2%)的B,培养基上,诱导出愈伤组织,在25±2℃条件下进行固体、液体培养、收集悬浮培养20天的细胞进行化学抽提和分离,得到一种橙黄色结晶,经 HPLC、UV、IR、MS 和 NMR 等分析鉴定,证明为药根碱。经药理试验表现有明显的生理活性。 The leaves of Blattella japonicus were induced to induce calli on medium containing 2,4-D (1 mg/l), KT (0.1 mg/l), agar (0.8%), and sucrose (2%) in medium B. Tissues were solid-state, liquid-cultured at 25±2°C, and cells collected in suspension culture for 20 days were chemically extracted and separated to obtain an orange-yellow crystal that was identified by HPLC, UV, IR, MS, and NMR. Demonstrated as jatrorrhizine. The pharmacological test showed obvious physiological activity.