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目的探讨氯通道开放在Ｔ淋巴细胞增殖过程中的作用。方法新鲜分离成人外周血Ｔ淋巴细胞，以ＣｏｎＡ诱导其增殖，用［３Ｈ］ＴｄＲ参入法测定并比较氯通道阻断剂ＤＩＤＳ、ＮＰＰＢ、９ＡＣ、ＮＦＡ、Ｆｕｒｏｓｅｍｉｄｅ对Ｔ淋巴细胞增殖的作用；测定钙通道阻滞剂ＳＫ＆Ｆ９６３６５、硝苯吡啶以及免疫抑制剂环孢霉素Ａ（ＣｓＡ）对Ｔ淋巴细胞增殖的作用；比较ＤＩＤＳ、ＮＰＰＢ与ＳＫ＆Ｆ９６３６５或ＣｓＡ的相互作用。结果９ＡＣ，ＮＦＡ，Ｆｕｒｏｓｅｍｉｄｅ对Ｔ细胞增殖无明显抑制作用；ＤＩＤＳ、ＮＰＰＢ呈浓度依赖性地抑制Ｔ细胞增殖，ＩＣ５０分别为１６８２３±３３６和（１２４３５±４２４）μｍｏｌ·Ｌ－１，并显著增强ＳＫ＆Ｆ９６３６５及ＣｓＡ抑制Ｔ细胞增殖的作用，且ＮＰＰＢ增强抑制作用强于ＤＩＤＳ。结论阻断氯通道可抑制ＣｏｎＡ诱导的Ｔ淋巴细胞增殖活化，氯通道开放影响细胞钙运动。
Objective To explore the role of chloride channel opening in the proliferation of T lymphocytes. Methods T lymphocytes were freshly isolated from adult peripheral blood and proliferated with ConA. The effect of chloride channel blockers DIDS, NPPB, 9AC, NFA and Furosemide on the proliferation of T lymphocytes was measured by [3H] TdR incorporation method. The effect of calcium channel blocker SK & F96365, nifedipine and immunosuppressant cyclosporin A (CsA) on the proliferation of T lymphocytes was tested. The interaction between DIDS, NPPB and SK & F96365 or CsA was also compared. Results 9AC, NFA and Furosemide had no obvious inhibitory effect on the proliferation of T cells. DIDS and NPPB inhibited the proliferation of T cells in a concentrationdependent manner with IC50 values of 16823 ± 336 and 12435 ± 424, respectively μmol·L-1, and significantly enhanced the effect of SK & F96365 and CsA on the proliferation of T cells. The inhibition of NPPB was stronger than that of DIDS. Conclusion Blocking chloride channel can inhibit ConA-induced T lymphocyte proliferation and activation, and opening of chloride channel can affect calcium movement.