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本实验研究了人重组ＧＭＣＳＦ对Ｕ９３７人单核细胞样细胞系ＨＬＡ和ＣＤ８６分子表达的调节作用。将Ｕ９３７细胞在ｒＧＭＣＳＦ１０μｇ／Ｌ培养１ｄ，ＨＬＡＤＲ分子的表达率为８３％，对照组为９１％；培养２ｄ，表达率为５１％，对照组为７８％；培养５ｄ，表达率为５３％，对照组为８１％。Ｕ９３７细胞在ｒＧＭＣＳＦ１０μｇ／Ｌ浓度下培养１ｄ，ＣＤ８６分子表达率为２％，对照组为１．６％；培养３ｄ，表达率为３．９１％，对照组为１％；培养５ｄ，表达率为５３２％，对照组为０．５８％。而ＭＨＣⅠ类分子表达率在１０μｇ／Ｌ浓度下培养５ｄ时，实验组和对照组均为１００％。以上结果显示，ｒＧＭＣＳＦ对Ｕ９３７细胞具有下调ＭＨＣⅡ类分子表达和上调ＣＤ８６分子表达的作用。ＭＨＣⅡ类分子和ＣＤ８６分子是Ｔ细胞重要的活化信号分子，因此可以说明，ｒＧＭＣＳＦ对Ｔ细胞参与的免疫应答具有重要的调节意义。
This study investigated the regulation of human recombinant GM-CSF on U937 human monocytoid cell line HLA and CD86 expression. The expression rate of HLADR in U937 cells was 83% in the control group, 51% in the control group, 51% in the control group, and 5% in the control group 53% in the control group and 81% in the control group. U937 cells were cultured in rGMCSF 10μg / L for 1 day. The expression rate of CD86 was 2% in control group and 1.6% in control group. The expression rate was 3.91% in 3d and 1% in control group. The rate was 532% and that of the control group was 0.58%. The expression of MHC Ⅰ class molecules at 10μg / L concentration for 5 days, the experimental group and the control group were 100%. The above results show that rGM-CSF on U937 cells have down-regulated expression of MHC Ⅱ molecules and up-regulate the expression of CD86 molecules. MHC Ⅱ class molecules and CD86 molecules are important T-cell activation signal molecules, it can be demonstrated that rGM CSF on T cells involved in the immune response has an important regulatory significance.