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目的研究维甲酸（ｒｅｎｔｉｎｏｉｃａｃｉｄ，ＲＡ）诱导的视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｌｉｕｍ，ＲＰＥ）细胞凋亡特征。方法体外培养的ＲＰＥ细胞中加入１０－５、１０－６、１０－７ｍｏｌ／ＬＲＡ，应用吖啶橙荧光染色和核苷酸末端转移酶介导的ｄＵＩＰ末端标记法（ＴｄＴ－ｍｅｄｉａｔｅｄｄＵＴＰｎｉｃｋｅｎｄｌａｂｅｌｉｎｇｍｅｔｈｏｄ，ＴＵＮＥＬ法）观察凋亡细胞。结果１０－５、１０－６、１０－７ｍｏｌ／ＬＲＡ诱导ＲＰＥ细胞凋亡，凋亡细胞表现为细胞固缩，染色质凝聚。ＴＵＮＥＬ法显示凋亡细胞ＤＮＡ片断化。１０－７、１０－６、１０－５ｍｏｌ／ＬＲＡ作用５天时，凋亡指数分别为３６．９％、４４．９％、６１．４％。作用６天时，凋亡指数分别为４８．０％、５９．９％、７４．２％。经统计学处理，同一ＲＡ浓度时，随作用时间延长凋亡指数增加；同一作用时间时，随ＲＡ浓度增大凋亡指数增加，差异有显著性（Ｐ＜０．０５）。结论ＲＡ能诱导ＲＰＥ细胞凋亡，且有很好的时间剂量效应
Objective To study the apoptosis of retinal pigment epithelial cells (RPE) induced by RA (rentinoic acid). Methods The RPE cells cultured in vitro were treated with 10 -5, 10 -6, 10-7 mol / L RA. Fluorescent staining with acridine orange and TdT-mediated dUTP nick end labeling method (TUNEL) Apoptotic cells were observed. Results 10-5,10-6,10-7 mol / LRA induced apoptosis of RPE cells, apoptotic cells showed cell shrinkage, chromatin condensation. TUNEL method showed apoptotic cell DNA fragmentation. The apoptotic indexes were 36.9%, 44.9% and 61.4% respectively after treated with 10-7,10-6,10-5 mol / LRA for 5 days. After 6 days, the apoptotic indexes were 48.0%, 59.9% and 74.2% respectively. The statistical analysis showed that the apoptotic index increased with the prolongation of action time at the same concentration of RA. There was a significant difference (P <0.05) with the increase of RA concentration at the same time. Conclusion RA can induce apoptosis of RPE cells with good time dose effect