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人巨细胞病毒(HCMV),已证实在体外有致癌潜能,具刺激细胞DNA合成和细胞增殖的能力。HCMV感染细胞后能产生与经生长因子刺激相似的反应:三磷酸肌醇、二乙酰甘油水平的增加,Ca~(2+)通透性增加,胞浆中Ca~(2+)活性及cAMP水平上升。已灭活的HCMV,不能产生极早(IE)和早期病毒抗原的合成,在其刺激的细胞增殖中具重要作用。这些都说明有缺陷的或灭活的HCMV颗粒也具启动细胞增殖的能力。本文作者希望证明HCMV是否影响某些原癌基因的表达。
Human cytomegalovirus (HCMV) has been demonstrated to have oncogenic potential in vitro, with the ability to stimulate cellular DNA synthesis and cell proliferation. HCMV can infect cells with a similar response to stimulation by growth factors: inositol triphosphate, increased diacetyl glycerol, increased Ca 2+ permeability, cytoplasmic Ca 2+ activity and cAMP Rise. Inactivated HCMV, which does not produce very early (IE) and early viral antigen synthesis, plays an important role in stimulating cell proliferation. All of these indicate that defective or inactivated HCMV particles also have the ability to initiate cell proliferation. The authors hope to prove whether HCMV affects the expression of certain proto-oncogenes.