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目的:探讨切割穹窿海马伞去神经支配大鼠海马内转移抑制蛋白1(metastasis suppressor 1,MTSS1)的表达变化。方法:SD大鼠随机分成正常组和切割穹窿海马伞后1、3、5、7、14 d组,采用Real-time PCR和免疫荧光组织化学技术观察穹窿海马伞切割后海马内MTSS1 mRNA的表达变化和MTSS1阳性细胞的分布情况。结果:(1)Real-time PCR结果显示:海马内MTSS1 mRNA的相对表达量在切割后3 d开始升高(P<0.05),5 d达到最高水平(P<0.01),7 d恢复至正常水平;(2)免疫荧光组织化学染色结果显示:MTSS1阳性细胞主要表达于海马齿状回门区及颗粒下层中,切割后5 d MTSS1阳性细胞数开始增多(P<0.01),7 d继续增多并达到高峰(P<0.01),14 d时开始下降至正常水平。结论:结合本课题组以往的工作,上述结果提示切割穹窿海马伞后MTSS1的高表达可能与海马神经再生过程中神经干细胞向神经元的分化有关。
Objective: To investigate the expression of metastasis suppressor 1 (MTSS1) in the hippocampus of the denervated fimbria fornix transected rats. Methods: SD rats were randomly divided into normal group and 1, 3, 5, 7, 14 d groups after fimbria fornix transection. Real-time PCR and immunofluorescence staining were used to observe the expression of MTSS1 mRNA in the hippocampus after fimbria fornix transection Changes and distribution of MTSS1-positive cells. Results: (1) Real-time PCR results showed that the relative expression of MTSS1 mRNA in hippocampus began to increase 3 days after cutting (P <0.05), reached the highest level on the 5th day (P <0.01) and returned to normal on the 7th day (2) The results of immunofluorescence staining showed that MTSS1 positive cells were mainly expressed in the dentate gyrus and granulosa subp., And the number of MTSS1 positive cells began to increase 5 days after the cut (P <0.01), and continued to increase on the 7th day And reached the peak (P <0.01), 14 d began to decline to normal levels. Conclusion: Combined with the previous work of our group, the above results suggest that the high expression of MTSS1 after the fimbria fornix transection may be related to the differentiation of neural stem cells into neurons during hippocampal neurogenesis.