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乙型脑炎病毒减毒活疫苗SA14-14-2株是由毒株SA14在原代地鼠肾细胞和小鼠中多次传代获得。SA14-14-2株已作为疫苗在中国人和猪中应用。为比较SA14与SA14-14-2株间的差异,作者检查了2株的血凝活性和电泳迁移率。将10~6PFU的SA14和SA14-14-2株分别注入小鼠,经过一定时间取脑组织,在Hank’s液内匀浆,离心收取悬液,用BHK-21单层细胞以空斑形成试验
Japanese encephalitis virus live attenuated vaccine strain SA14-14-2 strain SA14 primary mouse kidney cells and mice in many passages obtained. SA14-14-2 strain has been used as a vaccine in Chinese and pigs. To compare the differences between SA14 and SA14-14-2 strains, the authors examined the 2 strains for hemagglutination activity and electrophoretic mobility. 10 ~ 6PFU of SA14 and SA14-14-2 strains were injected into mice, brain tissue was taken after a certain time, homogenized in Hank’s solution, the suspension was collected by centrifugation, with BHK-21 monolayer cells to plaque formation test