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登革病毒可分为4个不同的血清型。通常,确定登革病毒血清型的方法主要依靠病毒分离及型特异性单克隆抗体的免疫荧光染色技术,但其耗时多,且成功率较低。新近,多聚酶链反应(PCR)已被广泛地应用于许多传染性疾病的诊断。为建立一种登革病毒分型的PCR方法,作者设计并合成了4对型特异性引物和一对共同引物。自登革病毒1～4型分别感染的C6/3b细胞及登革热病人血清中提取RNA,加入含0.15μmol/L引物、0.2mmol/L dNTP、10u
Dengue virus can be divided into 4 different serotypes. In general, the method for determining dengue serotypes relies primarily on immunofluorescence staining of virus-isolated and type-specific monoclonal antibodies, but it is time-consuming and has a low success rate. Recently, polymerase chain reaction (PCR) has been widely used in the diagnosis of many infectious diseases. To establish a PCR method for dengue virus typing, the authors designed and synthesized 4 pairs of type-specific primers and a pair of common primers. RNA was extracted from the sera of C6 / 3b cells and dengue fever patients infected with Dengue virus type 1 to 4, and the cells were treated with 0.15μmol / L primer, 0.2mmol / L dNTP, 10u