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[目的]观察青蒿琥酯对不同急性白血病细胞株的增殖抑制作用及凋亡机制探讨。[方法]应用不同浓度青蒿琥酯作用U937、HL60、Jurkat细胞株,MTT法检测抑制率;细胞形态学、DNA琼脂糖凝胶电泳、流式细胞术检测细胞凋亡;Western-blot法检测细胞凋亡过程中Bcl-2、Bax、Caspase 8和ICAD蛋白表达的变化。[结果]青蒿琥酯能够明显抑制U937、HL60、Jurkat细胞增殖;8μg/ml以上浓度青蒿琥酯作用三种细胞24h、48h、72h抑制率均为Jurkat〉HL60〉U937(均P<0.01);其增殖抑制作用与诱导细胞凋亡有关;随药物浓度的增高Bcl-2和ICAD蛋白表达量下降,而Bax蛋白表达上调,Caspase8蛋白出现明显的剪切激活带。[结论]青蒿琥酯既能通过线粒体途径又能通过外源性途径诱导急性白血病细胞凋亡,对Jurkat的抑制最强。
[Objective] To observe the inhibitory effect of artesunate on the proliferation of different acute leukemia cell lines and explore the mechanism of apoptosis. [Methods] The cell line U937, HL60 and Jurkat were treated with different concentrations of artesunate. The inhibitory rates were determined by MTT assay. Cell morphology, DNA agarose gel electrophoresis and flow cytometry were used to detect the cell apoptosis. Western-blot Changes of Bcl-2, Bax, Caspase 8 and ICAD Protein Expression during Apoptosis. [Results] Artesunate significantly inhibited the proliferation of U937, HL60 and Jurkat cells. The inhibition rates of artesunate at concentrations above 8 μg / ml for 24h, 48h and 72h were Jurkat> HL60> U937 (all P <0.01) ). The inhibition of proliferation was related to the induction of apoptosis. The expression of Bcl-2 and ICAD protein decreased with the increase of drug concentration, while the expression of Bax protein was up-regulated, and the cleavage activation band of Caspase8 protein appeared. [Conclusion] Artesunate can induce acute leukemia cell apoptosis both through mitochondrial pathway and extrinsic pathway, with the strongest inhibition on Jurkat.