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建立乳腺癌模型,了解游离的131I-bcl-2/bcl-xlASON(FA)以及阴离子长循环脂质体包裹的131I-bcl-2/bcl-xlASON(NA)在瘤鼠体内组织分布。SD大鼠尾静脉注射N-甲基亚硝基脲溶液,诱导建立乳腺癌模型。NA和FA分别静脉注射后0.5、1、2、3、4、6、12和24 h,断头处死瘤鼠,取其组织器官测量,计算每克组织放射性计数占注入剂量的百分数(%ID/g)。计算瘤鼠肿瘤/血液或肿瘤/肌肉比值。分别静脉注射0.4 mCi NA1、31I-错配序列阴离子长循环脂质体(NS)和131I-无义序列阴离子长循环脂质体(NN)后0.5、1、2、3、6和12 h,进行瘤鼠全身前后位静态显像。MNU致瘤时间为(96±1.2)d,SD大鼠致癌率为70%(90/130),组织学类型为乳腺癌。注射后10 h,NA在瘤鼠肿瘤组织、肝和脾内的分布分别为(6.23±0.23)%ID/g、(12.00±0.26)%ID/g和(18.25±1.33)%ID/g。肿瘤/血液和肿瘤/肌肉比值分别为6.29±0.76和10.55±0.68。NA注射后10 h,肿瘤显示最佳。NS和NN注射组瘤鼠肿瘤显示不清。NA体内清除率低,体内循环时间长,肿瘤部位浓聚增加,提示NA很可能提高放射反义治疗疗效。
The breast cancer model was established to investigate the tissue distribution of 131I-bcl-2 / bcl-xlASON (FA) and 131I-bcl-2 / bcl-xlASON (NA) N-methylnitrosourea solution was injected into the tail vein of SD rats to induce the establishment of breast cancer model. At 0.5, 1, 2, 3, 4, 6, 12, and 24 h after NA and FA administration, tumor-bearing mice were decapitated and their tissue and organ weights were measured. The percentage of radioactive counts per gram / g). Tumor / tumor or blood / tumor / muscle ratio was calculated. 0.5, 1, 2, 3, 6 and 12 h after intravenous injection of 0.4 mCi NA1 and 31I-mismatched anion long circulating liposomes (NS) and 131I nonsense anion long circulating liposomes (NN) The tumor before and after the whole body static imaging. The tumorigenic time of MNU was (96 ± 1.2) d. The carcinogenesis rate of SD rats was 70% (90/130). The histological type was breast cancer. The distribution of NA was (6.23 ± 0.23)% ID / g, (12.00 ± 0.26)% ID / g and (18.25 ± 1.33)% ID / g respectively at 10 h after injection in tumor tissue, liver and spleen. Tumor / blood and tumor / muscle ratios were 6.29 ± 0.76 and 10.55 ± 0.68, respectively. At 10 h after NA injection, the tumors showed the best. Neoplasms in NS and NN injected groups showed unclear tumors. NA in vivo clearance rate is low, the body cycle time is long, the accumulation of tumor sites increased, suggesting that NA is likely to improve the efficacy of radiation antisense therapy.