论文部分内容阅读
目的:观察HH胶囊的抗乙肝病毒(hepatitis B virus,HBV)疗效及作用机制。方法:采用大连依利特ODS-BP C18色谱柱研究HH胶囊的HPLC指纹图谱。HBV转基因小鼠随机分4组,苦参素组小鼠按150 mg·kg~(-1)·d~(-1)给予苦参素混悬液,高、低剂量HH胶囊组小鼠分别按7,2 g·kg~(-1)·d~(-1)给予HH混悬液,模型组给予等体积的生理盐水,5周后,酶联免疫吸附测定(ELISA)法检测血清乙型肝炎病毒表面抗原(hepatitis B virus surface antigen,HBs Ag),干扰素α(interferonα,IFN-α),干扰素β(interferonβ,IFN-β)及肝组织HBs Ag,常规苏木素-伊红(HE)染色观察肝组织病理变化,实时荧光定量聚合酶链式方应(q PCR)法检测血清及肝组织乙肝病毒的脱氧核糖核酸(hepatitis B virus DNA,HBVDNA)。结果:标定14个特征峰构成HH胶囊的指纹图谱,其中1号峰为没食子酸,8号峰为柯里拉京,9号峰为虎杖苷,10号峰为鞣花酸,13号峰为甘草酸,14号峰为齐墩果酸。与模型组比较,高剂量HH胶囊可以明显降低HBV转基因小鼠肝脏和血清中的HBs Ag,HBVDNA水平,差异具有统计学意义(P<0.01),与苦参素组比较差异无统计学意义;高剂量HH胶囊可显著升高小鼠IFN-α,IFN-β,差异有统计学意义(P<0.01,P<0.05)。结论:HH胶囊具有确切的抗HBV作用,可能与升高IFN-α,IFN-β有关。
Objective: To observe the curative effect and mechanism of HH capsule on hepatitis B virus (HBV). Methods: The HPLC fingerprints of HH capsule were studied by Dalian Ellite ODS-BP C18 column. HBV transgenic mice were randomly divided into 4 groups. The mice in the oxymatrine group were given the oxymatrine suspension at 150 mg · kg -1 (-1) d -1, and the mice in the high and low dose HH capsules groups The HH suspension was administered at 7 g · kg -1 · d -1, and the model group was given an equal volume of normal saline. After 5 weeks, serum B was detected by enzyme linked immunosorbent assay (ELISA) Hepatitis B virus surface antigen (HBsAg), interferonα (IFN-α), interferonβ (IFN-β) and liver tissue HBsAg, conventional hematoxylin-eosin ) Were used to observe the pathological changes of liver tissue. HBV DNA (HBV DNA) in serum and liver tissues were detected by real-time fluorescence quantitative polymerase chain reaction (q PCR). Results: The fingerprint of 14 HH capsules was calibrated. Among them, the first peak was gallic acid, the eighth peak was Corilagin, the ninth peak was polydatin, the tenth peak was ellagic acid, the third peak was licorice Acid, No. 14 peak for oleanolic acid. Compared with model group, high dose HH capsules could significantly reduce the levels of HBsAg and HBVDNA in liver and serum of HBV transgenic mice, the difference was statistically significant (P <0.01), but there was no significant difference compared with the matrine group; High-dose HH capsule can significantly increase the mice IFN-α, IFN-β, the difference was statistically significant (P <0.01, P <0.05). Conclusion: HH capsule has definite anti-HBV effect, which may be related to the increase of IFN-α and IFN-β.