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由于人乳头瘤病毒(HPV)复杂、多样, 对其检测方法不仅要求能对30多种该病毒进行检测,而且可对病毒分型。经流行病学检测证明,用HPV通用引物多聚酶链反应(PCR)法能够对大多数型别的HPV进行可靠检测和准确分型。广泛应用的L_1区通用引物MY09和MY11为高度保守区,很有可能将新型病毒扩增出来,结合限制性酶切片段长度多态性分析(RFLP),可发现新型HPV。本研究应用该方法在大量HPV感染调查中发现了4株新型的HPV,并用根据新型病毒序列合成特异性寡核苷酸探针,进行了大量的流行病学调查和病毒致病性分析。
Due to the complexity and diversity of human papillomavirus (HPV), its detection method not only requires that more than 30 kinds of the virus be tested, but also the virus can be typed. The epidemiological tests show that HPV universal primer polymerase chain reaction (PCR) method for most types of HPV can be reliably detected and accurately typed. The widely used L_1 common primers MY09 and MY11 are highly conserved regions and are likely to amplify novel viruses. Combined with restriction fragment length polymorphism analysis (RFLP), novel HPV types can be found. In this study, we found 4 new HPV types in a large number of HPV infections. We also performed a number of epidemiological investigations and virus pathogenicity analyzes using specific oligonucleotide probes based on novel viral sequences.