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目的研究建立用石墨消解-等离子体发射光谱法测定血液中15种元素的检测方法。方法取1 ml血样与1.5 ml硝酸混合,于石墨消解仪中,在120℃下消解1 h,冷却定容至10 ml,利用等离子体发射光谱法对消解液中的15种元素进行检测。结果该方法线性关系良好,相关系数(r)为0.999 5~0.999 9,最低检出限为0.001 0 mg/L~0.063 0 mg/L,回收率为85.5%~107.6%,相对标准偏差(RSD)为0.33%~2.07%。另外,采用本法和微波消解-等离子体发射光谱法对标准样品进行处理和检测,钼元素因参考值低于本方法的检出限,检测结果偏差较大,其余14种元素的RSD分别为0.21%~8.91%、0.89%~10.2%。结论本方法具有高效、灵敏、准确、干扰少、精密度高、检出限低、线性范围宽、同时检测多元素等特点,适合于大批量血液样品中部分宏量元素和微量元素的测定。
Objective To establish a method for the determination of 15 elements in blood by graphite digestion-plasma emission spectrometry. Methods One ml of blood sample was mixed with 1.5 ml of nitric acid in a graphite digestion apparatus and digested at 120 ° C for 1 h. The volume of the sample was cooled and fixed to 10 ml. Fifteen elements in the digestion solution were detected by plasma emission spectrometry. Results The method showed a good linearity with a correlation coefficient (r) of 0.999 5 to 0.999 9, a minimum detectable limit of 0.001 0 mg / L to 0.063 0 mg / L, and a recovery of 85.5% -107.6%. The relative standard deviation (RSD) ) Is 0.33% ~ 2.07%. In addition, the standard sample was treated and detected by this method and microwave digestion-plasma emission spectrometry. Because the reference value of molybdenum was lower than the detection limit of this method, the detection results deviated greatly. The RSDs of the other 14 elements were 0.21% ~ 8.91%, 0.89% ~ 10.2%. Conclusion The method is characterized by high efficiency, sensitivity, accuracy, low interference, high precision, low detection limit, wide linear range and simultaneous detection of multiple elements. It is suitable for the determination of some macro elements and trace elements in large quantities of blood samples.