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将活化醇盐水解法制备的SiO_2纳米粒子分别与羰基还原酶(CR)和甲酸脱氢酶(FDH)进行共价固定化,固定化CR与FDH耦合,连续催化转化b-羟基苯乙酮制备(R)-苯基乙二醇,考察了NADH的再生与循环利用性.结果表明,纳米粒子固定化CR和FDH酶载量分别为3.32和5.55 mg/g,催化活性为游离酶的50%~60%,最适反应p H值分别为6.5和8.5,最适反应温度分别为40和45℃.耦合体系进行12批次反应,产物(R)-苯基乙二醇累积量达35.6 g/L,纳米粒子生产能力达178 g/g.纳米粒子固定化酶经简单离心收集后可重复利用.
The SiO 2 nanoparticles prepared by activated alkoxide hydrolysis were covalently immobilized with carbonyl reductase (CR) and formic acid dehydrogenase (FDH) respectively. The immobilized CR and FDH were coupled and the b-hydroxyacetophenone R) -phenylglycol was used to investigate the regeneration and recycle of NADH.The results showed that the loadings of immobilized CR and FDH were 3.32 and 5.55 mg / g, respectively, and the catalytic activity was 50% 60%, the optimal reaction p H values were 6.5 and 8.5, respectively, and the optimum reaction temperature was 40 and 45 ℃, respectively.The coupling system was operated in 12 batches and the product (R) -phenyl glycol reached 35.6 g / L, nanoparticle production capacity of 178 g / g. Nanoparticles immobilized enzyme can be reused after simple centrifugation collected.