论文部分内容阅读
采用一种简化了的Bjorklund方法,直接根据荧光团的表观激发光谱特征对豚鼠腹腔神经节SIF细胞内儿茶酚胺(CA)的性质进行鉴别,在进行了甲醛诱发荧光(FIF)反应,随后用盐酸处理5分钟后,SIF细胞内的CA荧光团的最高激发峰由420nm变为350nm;390/350nm激发峰比值也降至1以下。这一变化与在同一条件下在标准蛋白膜上记录的去甲肾上腺素(NA)荧光团的特征是一致的;而与多巴胺荧光团的光谱行为有明显差异。结果表明,这些SIF细胞中的CA主要是NA。
Using a simplified Bjorklund method, the properties of catecholamine (CA) in guinea pig celiac ganglion SIF cells were directly identified based on the apparent excitation spectra of the fluorophores. Formaldehyde-induced fluorescence (FIF) reactions were performed followed by treatment with hydrochloric acid After 5 minutes of treatment, the maximum excitation peak of CA fluorophores in SIF cells changed from 420 nm to 350 nm; the ratio of 390/350 nm excitation peak also dropped below 1. This change is consistent with the characteristic of norepinephrine (NA) fluorophores recorded on standard protein membranes under the same conditions; however, there is a significant difference from the spectral behavior of dopamine fluorophores. The results show that CA in these SIF cells is predominantly NA.