目的和方法 :观察缺氧、复氧对人心房肌细胞内钙离子浓度的影响。急性分离人心房肌细胞 ,以Fluo- 3作为Ca2 +指示剂 ,用激光共聚焦显微镜观察细胞内Ca2 +浓度变化。结果 :人心房肌细胞在缺氧前[Ca2 +]i 为 (117± 48)nmol/L。在缺氧 15min时 ,[Ca2 +]i 即增加为 (187± 6 4)nmol/L(P <0 .0 5 ,vs缺氧前 )。缺氧30min时 [Ca2 +]i 为 (4 17± 139)nmol/L(P <0 .0 1,vs缺氧前 )。缺氧后复氧 30min[Ca2 +]i 为 (786± 2 38)nmol/L(P<0 .0 1,vs缺氧前 )。结论 :人心房肌细胞在缺氧状态下胞内Ca2 +浓度升高 ,而当其短期复氧时 ,胞内Ca2 +浓度继续增加 Objective and Methods: To observe the effect of hypoxia and reoxygenation on calcium ion concentration in human atrial myocytes. Acute isolation of human atrial myocytes, Fluo-3 as a Ca2 + indicator, confocal laser scanning microscope intracellular Ca2 concentration changes. Results: [Ca2 +] i in human atrial myocytes was (117 ± 48) nmol / L before hypoxia. At 15 min of hypoxia, [Ca2 +] i increased to (187 ± 6 4) nmol / L (P <0.05, vs hypoxia). [Ca2 +] i was (4 17 ± 139) nmol / L at 30 min hypoxia (P <0.01, vs hypoxia). Oxygen reoxygenation after 30min [Ca2 +] i was (786 ± 238) nmol / L (P <0.01, vs hypoxia). CONCLUSION: Intracellular Ca2 + concentration is increased in human atrial myocytes under hypoxia, whereas intracellular Ca2 + concentration continues to increase in short-term reoxygenation