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目的:探讨化疗前后乳腺癌患者血清中IL-6、IL-8及TNF-α的变化及意义。方法:选择住院手术乳腺癌术后1个月、化疗1个疗程(21天)患者50例,化疗前、后均静脉采血,同期选取体检健康女性30例作为对照组亦静脉采血,均留取血清待测。采用ELISA酶联免疫法检测IL-6、IL-8及TNF-α的蛋白水平,采用流式细胞免疫学法检测IL-6、IL-8及TNF-α的细胞阳性百分率。结果:与对照组比较,化疗前乳腺癌组患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率均升高,差异有统计学意义(P<0.01);化疗后患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率均略升高,差异无统计学意义(P>0.05);化疗前乳腺癌组患者血清中IL-6、IL-8、TNF-α的蛋白水平及细胞阳性百分率明显高于化疗后患者,差异有统计学意义(P<0.01)。结论:化疗能够通过降低乳腺癌患者血清中IL-6、IL-8、TNF-α的水平抑制肿瘤的浸润和转移,这为乳腺癌的临床治疗提供新的靶点。
Objective: To investigate the changes and significance of serum IL-6, IL-8 and TNF-α in patients with breast cancer before and after chemotherapy. Methods: One month after surgery for breast cancer, 50 patients were treated with chemotherapy for one course of treatment (21 days). Blood samples were obtained from venous blood before and after chemotherapy. 30 healthy women were selected as the control group during the same period. Serum to be tested. The protein levels of IL-6, IL-8 and TNF-αwere detected by enzyme linked immunosorbent assay (ELISA). The positive percentage of IL-6, IL-8 and TNF-α were detected by flow cytometry. Results: Compared with the control group, the serum levels of IL-6, IL-8 and TNF-α in patients with pre-chemotherapy breast cancer were significantly increased (P <0.01); after chemotherapy Serum levels of IL-6, IL-8 and TNF-α in patients with breast cancer slightly increased (P> 0.05). The levels of IL-6, IL -8, TNF-α protein level and the positive percentage of cells was significantly higher than that after chemotherapy, the difference was statistically significant (P <0.01). Conclusion: Chemotherapy can inhibit tumor invasion and metastasis by decreasing the levels of IL-6, IL-8 and TNF-α in the serum of breast cancer patients, which provides a new target for the clinical treatment of breast cancer.