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从部分减毒的麻疹病毒Ｌ４株感染的Ｖｅｒｏ细胞中提取ｍＲＮＡ、进行逆转录及ＰＣＲ扩增。克隆到了长１．９ｋｂ的４个血凝素基因克隆。ＤＮＡ序列分析发现，Ｌ４株的血凝素基因有４个碱基与Ｅｄｍｏｎｓｔｏｎ株不一致，造成３个氨基酸差异。这４个克隆在重组痘苗中表达后，３个克隆的表达产物均表现出良好的血凝活性、助融合活性（Ｆｕｓｉｏｎｈｅｌｐｅｒ）和较强的免疫原性。表达血凝素分子量分别为未糖化的６８ｋＤ以及糖化的７９ｋＤ分子，免疫的兔血清显示出较高的ＥＬＩＳＡ（１：８００）～１：３２００）、ＨＩ（１：１６０－：１：１２８０）、ＨＬＩ（１：１６０～１：６４０）及ＮＴ（１：１００～１：４８６）滴度。麻疹病毒ＬＡ株的血凝素基因的克隆为麻疹重组疫苗的研究提供了重要材料。
MRNA was extracted from Vero cells infected with a partially attenuated measles virus L4 strain for reverse transcription and PCR amplification. Four hemagglutinin gene clones with a length of 1.9 kb were cloned. DNA sequence analysis found that L4 strain hemagglutinin gene has 4 bases and Edmonston strains are inconsistent, resulting in three amino acid differences. After the four clones were expressed in recombinant vaccinia, all three clones showed good hemagglutination activity, Fusionhelper activity and strong immunogenicity. The expressed hemagglutinin molecular weights were non-glycated 68 kD and glycosylated 79 kD molecules, respectively, and the immunized rabbit sera showed higher ELISA (1: 800) to 1: 3200), HI (1: 160 to 1: 1280) HLI (1: 160 ~ 1: 640) and NT (1: 100 ~ 1: 486) titer. The cloning of the hemagglutinin gene of measles virus LA strain provided important materials for the study of recombinant measles vaccine.