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目的了解中山地区铜绿假单胞菌氨基糖苷类药物的耐药表型和基因表型的分布及其关系。方法采用VITEK-2Compact高级专家系统判定2010年1-9月896株铜绿假单胞菌对氨基糖苷类药物的耐药表型,并对其中15株多药耐药铜绿假单胞菌采用PCR的方法检测4种氨基糖苷类修饰酶基因。结果 896株铜绿假单胞菌及15株多药耐药铜绿假单胞菌对氨基糖苷类药物的耐药表型模式均主要为表型模式RESISTANT(GEN、NET、AMI、TOB),阳性株分别为468、12株;15株多药耐药菌株全部检出氨基糖苷类耐药基因:aac(6′)-Ⅰ、aac(6′)-Ⅱ、ant(2″)-Ⅰ、ant(3″)-Ⅰ分别为13、4、3、8株。结论临床分离的铜绿假单胞菌以单一耐药表型模式为主,推测该地区氨基糖苷类药物耐药的主要原因,是氨基糖苷类修饰酶基因的存在导致。
Objective To understand the distribution and relationship of drug resistance phenotypes and gene phenotypes of Pseudomonas aeruginosa aminoglycosides in Zhongshan area. Methods The VITEK-2Compact expert system was used to determine the resistance phenotypes of aminoglycosides in 896 Pseudomonas aeruginosa strains from January to September in 2010, and 15 strains of multidrug-resistant Pseudomonas aeruginosa were analyzed by PCR Methods Four aminoglycoside modified enzyme genes were detected. Results The resistant phenotype patterns of aminoglycosides in 896 strains of Pseudomonas aeruginosa and 15 strains of multidrug-resistant Pseudomonas aeruginosa were RESISTANT (GEN, NET, AMI, TOB), positive Were 468 and 12 strains, respectively. A total of 15 multidrug resistant strains were identified as aminoglycoside resistance genes: aac (6 ’) - Ⅰ, aac (6’) - Ⅱ, ant 3 ") - Ⅰ were 13,4,3,8 strains. Conclusions The clinical isolates of Pseudomonas aeruginosa are mainly single drug resistant phenotypes. It is speculated that the main reason of aminoglycoside resistance in this area is the existence of aminoglycoside modifying enzyme gene.