论文部分内容阅读
测定了黑曲霉葡萄糖淀粉酶(E.C.3.2.1.3)在三种改性的、具有中孔和大孔的分子筛上的吸附等温线并将吸附量和吸附等温线的形状与分子筛的等电点、孔容、孔径及酸性相关联.讨论了孔结构和不同酶吸附量对分子筛固定化葡萄糖淀粉酶活力的影响.发现葡萄糖淀粉酶在再造孔分子筛上的单层饱和吸附量与再造孔的方法密切有关,三种不同再造孔方法制得的分子筛具有不同的骨架Si/Al比、不同的孔分布和比表面积.不同的Si/Al比导致不同的酸性质和等电点.酶吸附量与载体的表面酸性、等电点以及吸附时溶液的pH有关,分子筛对酶的吸附以静电作用为主.其次,当中孔孔径和孔容越大时,单层饱和吸附量亦越大.随着分子筛对葡萄糖淀粉酶的吸附量增加,固定化酶的活力增大,但固定化酶的比活力随吸附量的增加、中孔孔容和孔径的减小而下降.
The adsorption isotherms of Aspergillus niger glucoamylase (EC 3.2.1.3) on three modified mesopores with mesopores and macropores were determined and the adsorption isotherms and adsorption isotherms were correlated with the isoelectric point, Pore volume and acidity.The effects of pore structure and amount of different enzyme adsorption on the activity of molecular sieve immobilized glucoamylase were discussed.It was found that the single layer saturated adsorption amount of glucoamylase on reconstituted pore sieve was close to that of reconstituted pore The molecular sieves prepared by three different methods of re-pore preparation have different Si / Al ratios, different pore distributions and specific surface areas. Different Si / Al ratios lead to different acid properties and isoelectric points. Of the surface acidity, isoelectric point and pH of the solution on the adsorption, molecular sieve on the adsorption of the enzyme electrostatic mainly.Secondly, when the pore size and pore volume is larger, the greater the single-layer saturated adsorption capacity.With the molecular sieve The adsorption amount of glucoamylase increased and the activity of immobilized enzyme increased. However, the specific activity of immobilized enzyme decreased with the increase of adsorption amount and the decrease of pore volume and pore size.