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本实验室过去工作提示α脂蛋白在动脉粥样硬化中的作用,与以后Miller的观察一致。为此,我们进一步在实验性动脉粥样硬化形成过程中,着重比较α脂蛋白图谱及其与动脉粥样硬化的关系。 方法为本实验室过去建立的纸上电泳法。Whatman N01滤纸,巴比妥及其钠盐缓冲液PH8.6、离子强度0.05,电流5.5ma,电压250V,电泳时间3小时,完毕后100℃烤干,分别用偶氮胭脂红染蛋白、用
Past work in our laboratory suggests that alpha lipoprotein has a role in atherosclerosis, consistent with Miller’s observations. To this end, we further in the process of experimental atherosclerosis, focusing on the comparison of α-lipoprotein atlas and its relationship with atherosclerosis. Method for the laboratory in the past established on paper electrophoresis. Whatman N01 filter paper, barbital and sodium salt buffer PH8.6, ionic strength 0.05, current 5.5ma, voltage 250V, electrophoresis time 3 hours, after completion of drying at 100 ℃, respectively, with azo carmine stained protein, with