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肺泡巨噬细胞在吞噬异物和细菌过程中,或在酵母多糖等刺激后,能产生多种反应氧类(ROS),化学发光(CL)强度直接反映ROS的量,ROS不仅有强烈的杀菌功能,而且与免疫、衰老、炎症、癌症,类风湿性关节炎、缺血等有关,因而PAM的化学发光研究对探讨某些疾病发病机制,治疗将有一定意义。本文介绍了肺灌洗液(BAL)获取方法,肺泡巨噬细胞悬液的制备及化学发光测定方法,并对实验条件进行初步探讨。并应用化学发光方法测定64例肺泡巨噬细胞化学发光,其中正常人10例,患者54例,其中特发性肺纤维化、肺癌、肺炎,PAM的自发发光及刺激发光都显著高于正常对照组(P<0.01),哮喘、胸膜炎、结缔组织病与正常对照组也有区别(P<0.05),支扩、肺结核,结节病与正常对照组无统计学差异(P>0.05)。
Alveolar macrophages can produce a variety of reactive oxygen species (ROS) during phagocytosis of foreign bodies and bacteria, or after stimulation with zymosan. Chemiluminescence (CL) intensity directly reflects the amount of ROS. ROS not only has a strong bactericidal function , But also with the immune, aging, inflammation, cancer, rheumatoid arthritis, ischemia and so on, so the chemiluminescence study of PAM on the pathogenesis of certain diseases, treatment will be of some significance. This article describes the method of obtaining lung lavage fluid (BAL), preparation of alveolar macrophage suspension and determination of chemiluminescence, and preliminary study of experimental conditions. Chemiluminescence was used to determine the chemiluminescence of 64 alveolar macrophages, including 10 normal subjects and 54 patients. Among them, idiopathic pulmonary fibrosis, lung cancer, pneumonia, spontaneous luminescence and stimulated luminescence of PAM were significantly higher than those of normal control (P <0.01). Asthma, pleurisy and connective tissue disease were also different from normal control group (P <0.05). There was no significant difference between bronchiectasis, tuberculosis, sarcoidosis and normal control group (P> 0.05).