目的　探讨＋ ＧＺ作用后，大鼠心肌组织内内皮素－１（ＥＴ－１）和内皮型一氧化氮合酶（ｅＮＯＳ）ｍ ＲＮＡ 表达变化。　方法　 ２４只雄性Ｗｉｓｔａｒ 大鼠随机分成对照组、＋ ＧＺ重复暴露后３０ ｍ ｉｎ、６ ｈ 和２４ ｈ四组，每组６只。对照组大鼠Ｇ 值为＋ １ ＧＺ， 实验组大鼠在动物离心机上经历了３次＋ １０ ＧＺ／３ ｍ ｉｎ（两次中间间隔３０ ｍ ｉｎ）作用。分别于暴露后３０ ｍ ｉｎ、６ ｈ 和２４ ｈ 处死大鼠取心，提取总ＲＮＡ，用半定量反转录聚合酶链反应（ＲＴ－ＰＣＲ）检测方法检测大鼠心肌组织ＥＴ－１和ｅＮＯＳｍ ＲＮＡ 表达水平。　结果　＋ ＧＺ暴露后３０ ｍ ｉｎ 和６ ｈ 大鼠心肌组织ＥＴ－１ ｍ ＲＮＡ 明显升高，ｅＮＯＳｍ ＲＮＡ 明显降低，但２４ ｈ 时均恢复正常。　结论　＋ ＧＺ暴露可使大鼠心肌组织内ＥＴ－１和ｅＮＯＳｍ ＲＮＡ 表达发生改变，其在心肌损伤中可能起一定的作用，但这种变化是可逆的 Objective To investigate the expression of endothelin-1 (ET-1) and endothelial nitric oxide synthase (eNOS) m RNA in rat myocardium after + GZ treatment. Methods Twenty-four male Wistar rats were randomly divided into four groups: control group (n = 6) and control group (n = 6). The G value of the control group was + 1 GZ, and the rats in the experimental group underwent three +10 GZ / 3 mins (two intermediate intervals of 30 mins) on the animal centrifuge. The rats were sacrificed at 30 mins, 6 h and 24 h after exposure, respectively. The total RNA was extracted and the expression of ET-1 and eNOSm was detected by semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) RNA expression level. Results ET-1 m RNA in myocardial tissue of 30 min and 6 h after + GZ exposure was significantly increased, and eNOSm RNA was significantly decreased, but returned to normal after 24 h. Conclusion + GZ exposure can change the expression of ET-1 and eNOSmRNA in myocardial tissue of rats, which may play a role in myocardial injury, but this change is reversible