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钼(Mo)和硒(Se)都是人体必需微量元素,与某些心脏病、克山病病因及发病机制有关。但是,哪一个与上述疾病关系更密切说法不一。我们曾证明 Mo 和 Se 对培养心肌细胞生长发育和自发性收缩都有良好的促进作用,Se 比 Mo 更明显。为进一步验证这一结果,本实验利用细胞内微电极技术,对比观察二者对培养心肌细胞电生理特性的影响。其结果表明:Mo 和 Se对心肌细胞跨膜电位各项指标均有一定影响,其中具有统计学意义的是,动作电位时程延长;静息电位绝对值减小。这提示了,Mo 和 Se 可能与 Ca~(2+)、K~+通道的活性有关,对比之下,Se 比 Mo更明显。
Both molybdenum (Mo) and selenium (Se) are essential trace elements for the human body and are associated with some heart diseases, the etiology and pathogenesis of Keshan disease. However, which one is more closely related to the above diseases is not the same. We have demonstrated that Mo and Se can promote the growth and spontaneous contraction of cardiomyocytes, and Se is more obvious than Mo. In order to further verify this result, we used intracellular microelectrode technology to compare the effects of the two on the electrophysiological characteristics of cultured cardiomyocytes. The results showed that: Mo and Se on the transmembrane potential of myocardial cells have a certain impact on various indicators, of which statistically significant is that the action potential duration extended; absolute value of resting potential decreased. This suggests that Mo and Se may be related to the activity of Ca ~ (2 +) and K ~ + channels. In contrast, Se is more obvious than Mo.