在基因工程研究中,由于大肠杆菌遗传背景清楚,表达量高,因而做为工程细胞得到广泛应用。但同时也存在着一些问题,如内毒素污染问题难以解决。我们曾试图用多粘菌素B亲和层析法去除大肠杆菌表达的人重组白细胞介素-3(IL-3),但效果不理想。我们在用Rotofor等电聚焦仪纯化本室的基因工程产品时,发现内毒素聚焦于碱性范围内,因而可利用这一原理选 In genetic engineering, due to the genetic background of E. coli is clear, high expression, so as engineering cells are widely used. But there are also some problems, such as the problem of endotoxin pollution is difficult to solve. We have tried to use polymyxin B affinity chromatography to remove E. coli expression of human recombinant interleukin-3 (IL-3), but the effect is not satisfactory. When we used the Rotofor isoelectric focusing instrument to purify gene engineering products in our laboratory, we found that endotoxin was focused on the basic range, so we can use this principle to select