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目的:利用分子生物技术构建免疫球蛋白G(IgG)启动子调控绿色荧光蛋白(green fluorescent protein,GFP)转基因细胞,尝试以IgG基因表达起始为标准在临床前对中药注射剂进行其诱导II型变态反应的可能性预测。方法:构建IgG启动子调控绿色荧光蛋白表达的转基因细胞系,用葛根素,KCl,聚氧乙烯脱水山梨醇单油酸酯(吐温80)对该系统进行测试,并尝试用该系统对不同批次鱼腥草注射液进行筛选评价,观察统计各给药组中发光细胞数量的变化。结果:非离子表面活性剂吐温80对IgG表达启动过程有抑制作用,葛根素能显著激活IgG基因的表达,KCl对IgG表达无作用。所测批次鱼腥草注射液对IgG表达没有激活作用。结论:IgG启动子调控绿色荧光蛋白表达细胞模型能在IgG表达层次评价药物致敏性,吐温80能在转录水平上抑制IgG的表达,所测批次鱼腥草注射液在IgG转录层次上无诱导II型变态反应活性。
OBJECTIVE: To construct an immunoglobulin G (IgG) promoter to regulate green fluorescent protein (GFP) transgenic cells using molecular biotechnology, and to try to induce traditional Chinese medicine injection to induce type II preclinical IgG gene expression as a criterion. Prediction of the likelihood of allergy. METHODS: A transgenic cell line that regulates green fluorescent protein expression by an IgG promoter was constructed. The system was tested with puerarin, KCl, polyoxyethylene sorbitan monooleate (Tween 80), and the system was tried differently. A batch of Houttuynia Cordata Injection was screened for evaluation, and changes in the number of luminescent cells in each administration group were observed and counted. RESULTS: The non-ionic surfactant Tween 80 inhibited the initiation of IgG expression. Puerarin significantly activated IgG gene expression. KCl had no effect on IgG expression. The tested Houttuynia injections had no activating effect on IgG expression. CONCLUSION: The IgG promoter-regulated green fluorescent protein expression cell model can evaluate drug sensitization at the level of IgG expression. Tween 80 can inhibit the expression of IgG at the transcriptional level. Houttuynia injections tested at the IgG transcription level No induction of type II allergic activity.