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背景:越来越多的研究事实表明免疫系统不是一个仅有自主调节的孤立系统,而是与中枢神经系统之间存在双向联系。目的:探讨哮喘大鼠肺脏和大脑中c-fos表达的分布及其意义。设计:随机对照实验。单位:南方医科大学南方医院肿瘤科和珠江医院神经外科。材料:实验在2004-01/08在南方医科大学南方医院肿瘤科实验室和珠江医院神经外科完成。选择成年健康雄性大鼠14只。随机分为实验组10只和对照组4只。方法:实验组第1天用卵蛋白10mg加氢氧化铝干粉200mg与灭活百日咳菌苗5×109个配成2mL混悬液腹腔注射,于第15天开始用10g/L卵蛋白超声雾化吸入,2次/h,共3d,制作卵蛋白致敏大鼠哮喘模型,对照组第1天用生理盐水2mL腹腔注射,于第15天用生理盐水雾化吸入,30mL/d,共3d。所有大鼠在麻醉状态下灌流固定取肺和脑组织,采用免疫组织化学卵白素-生物素-过氧化物复合物法和图像分析等技术观察Fos蛋白在肺脏和大脑内的分布情况。主要观察指标:Fos蛋白在脑内不同区域和肺脏中的分布。结果:14只大鼠均进入结果分析。①哮喘组大鼠肺脏和大脑中c-fos表达明显高于对照组(P<0.05);Fos阳性产物在脑内主要集中分布在额顶皮质、边缘前脑(扣带皮质、梨状皮质和中央杏仁核等)、丘脑室旁核、下丘脑室旁核、视上核、下丘脑外侧区、下丘脑室周核、孤束核、最后区和延髓腹外侧区内;小脑内无明显Fos分布密集区。②哮喘组气道壁及肺组织中可见大量的c-fos阳性细胞,阳性炎症细胞主要分布于黏膜层、黏膜下层及平滑肌周围;而对照组动物气道及肺组织中无或偶见免疫反应极弱的c-fos阳性细胞。结论:哮喘大鼠不仅肺脏中c-fos表达明显增加,而且在延髓内脏带及其上行投射神经核团(下丘脑、杏仁核等)表达也较多,说明原癌基因c-fos表达可能与哮喘神经免疫调节密切相关。
BACKGROUND: More and more research facts show that the immune system is not an isolated system with independent regulation only, but a two-way relationship with the central nervous system. Objective: To investigate the distribution and significance of c-fos expression in lung and brain of asthmatic rats. Design: Randomized controlled experiment. Unit: Nanfang Hospital, Southern Medical University and Department of Neurosurgery, Zhujiang Hospital. MATERIALS: The experiment was performed at Department of Neurosurgery, Nanfang Hospital Cancer Institute, Southern Medical University and Department of Neurosurgery, Pearl River Hospital from January 2004 to January 2004. Fourteen healthy adult male rats were selected. Randomly divided into experimental group 10 and control group 4. Methods: On the first day of the experimental group, 200 mg of ovalbumin plus 200 mg of dry powder of aluminum hydroxide and 5 × 109 of inactivated B. pertussis vaccine were intraperitoneally injected into a 2 mL suspension. On the 15th day, 10 g / L ovalbumin was atomized Inhalation, 2 times / h, a total of 3d, making ovalbumin-sensitized rat asthma model, the control group on the first day with saline 2mL intraperitoneal injection, on the 15th day with saline inhalation, 30mL / d, a total of 3d. All rats were perfused and fixed in the lung and brain tissue under anesthesia. The distribution of Fos protein in the lung and brain was observed by immunohistochemical avidin-biotin-peroxide complex method and image analysis. MAIN OUTCOME MEASURES: Distribution of Fos protein in different regions of the brain and lungs. Results: All 14 rats entered the result analysis. ① The expression of c-fos in lung and brain of asthmatic rats was significantly higher than that of the control group (P <0.05). The positive products of Fos in the brain mainly distributed in frontal cortex, marginal forebrain (cingulate cortex, piriform cortex and Central amygdala, etc.), paraventricular nucleus of hypothalamus, paraventricular nucleus of hypothalamus, supraoptic nucleus, lateral hypothalamus, periventricular nucleus of hypothalamus, nucleus tractus solitarius, caudate nucleus and rostral ventrolateral medulla; Distribution area. ②A large number of c-fos positive cells were found in the airway wall and lung tissue of asthmatic group, the positive inflammatory cells were mainly distributed in the mucosa, submucosa and smooth muscle; while in the control group, there was no or no immune reaction in the airway and lung tissue Very weak c-fos positive cells. CONCLUSIONS: Not only the expression of c-fos in the lungs of asthmatic rats increased but also the expression of c-fos in the medullary visceral zone and its upward projection nucleus (hypothalamus, amygdala, etc.) Asthma is closely related to neuroimmunity regulation.