一、前言动物细胞组织培养法在医学领域里的研究,经过十余年的努力,有了很大的发展。尤其对人体恶性肿瘤组织的培养,早在六十年代,人们就利用 HeLa 细胞株在浮游状态下能够增殖的变异亚种(Subclone),开始进行了搅拌培养。但细胞培养也还存在着许多未能解决的问题,例如除 L 细胞等之外,在大多数动物细胞培养方面,都应用10%左右的牛血清,特别是牛胎儿血清。由于其批号不同,细胞增殖率差别亦大。此外,在有用物质的产生等方面,其重演性也存在着很多问题。同 I. Introduction Animal tissue culture in the field of medical research, after more than ten years of hard work, has made great progress. Especially for human malignant tumor tissue culture, as early as the 1960s, people began to carry out agitation culture by using the subclone of HeLa cell strain capable of proliferating in the floating state. However, there are still many unsolved problems in cell culture. For example, about 10% of bovine serum, especially fetal bovine serum, is used in most animal cell culture in addition to L cells. Due to the different lot numbers, cell proliferation rates also vary greatly. In addition, in the production of useful substances, there are many problems with its repetition. with