采用PCR技术，从细胞中获取粒细胞／巨噬细胞集落刺激因子cDNA，并在5′端设计上凝血酸酶切位点、5个组氨酸密码子、起始密码子及EcoR1酶切位点，在3′端设计上BamH1酶切位点。将扩增产物酶切后克隆到pBV220质粒的EcoRⅠ－BamHⅠ酶切位点，转化大肠杆菌DH5α，筛选阳性重组子（pGM09／DH5α）。在E.coli中获高效表达，表达量占总蛋白的31．2％，生物活性为1．1×107U／L发酵液，纯化工艺简化。 The PCR technique was used to obtain the granulocyte / macrophage colony stimulating factor cDNA from the cell, and the 5 ’end of the clotting site, 5 histidine codons, start codon and EcoR1 cleavage site Point, BamH1 restriction site was designed on the 3 ’end. The amplified product was digested and cloned into pBV220 plasmid EcoR Ⅰ-BamH Ⅰ restriction sites, transformed into E. coli DH5α, positive clones screened (pGM09 / DH5α). It was highly expressed in E.coli, accounting for 31.2% of the total protein. The biological activity was 1.1 × 107U / L, and the purification process was simplified.