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目的建立高效液相色谱法测定七味广枣丸中丁香酚、木香烃内酯和去氢木香内酯含量的方法。方法色谱柱为Agilent ZORBAX Eclipse SB-C18(4.6mm×150mm,5μm);流动相为5%乙腈-乙腈(58∶42);流速为1mL·min-1;检测波长程序:0～15.0min为280nm;15.1～40min为225nm。结果丁香酚,木香烃内酯和去氢木香内酯的保留时间分别约为6,21和24min,与相邻峰的分离度大于1.5。以峰面积对进样浓度(μg·mL-1)线性回归,丁香酚回归方程为Y=0.1045ρ-6.161,r=0.9999,线性范围0.5520～13.80μg;木香烃内酯回归方程为Y=0.09539ρ-0.2972,r=0.9999,线性范围0.07400～1.850μg;去氢木香内酯回归方程为Y=0.07851ρ-0.07949,r=0.9998,线性范围0.05950～1.488μg;丁香酚,木香烃内酯和去氢木香内酯的回收率分别为99.2%,101.3%和97.0%,RSD分别为0.89%、1.6%和1.6%。结论本方法操作简便,测定结果准确,重复性好,可用于七味广枣丸中丁香酚、木香烃内酯和去氢木香内酯含量的同时测定。
OBJECTIVE To establish a method for the determination of eugenol, ligroxolide and dehydrocostus lactone in Qiwei Guangzao Wan by high performance liquid chromatography. Methods The column was Agilent ZORBAX Eclipse SB-C18 (4.6mm × 150mm, 5μm). The mobile phase consisted of 5% acetonitrile-acetonitrile (58:42) and the flow rate was 1mL · min-1. 280nm; 15.1 ~ 40min is 225nm. Results The retention times of eugenol, woodlocolactone and dehydrocostuslactone were about 6, 21 and 24 min, respectively, with resolution of more than 1.5 with adjacent peaks. The regression equation of eugenol was Y = 0.1045ρ-6.161, r = 0.9999, and the linear range was 0.5520 ~ 13.80μg. The regression equation of woodlne lactone was Y = 0.09539 ρ-0.2972, r = 0.9999, linear range of 0.07400 ~ 1.850μg; dehydrogenation Cycloside lactone regression equation Y = 0.07851ρ-0.07949, r = 0.9998, linear range of 0.05950 ~ 1.488μg; The recoveries of esters and dehydrocostus lactones were 99.2%, 101.3% and 97.0%, respectively, with RSDs of 0.89%, 1.6% and 1.6%, respectively. Conclusion The method is simple, accurate and reproducible. It can be used for the simultaneous determination of eugenol, ligroxolone and dehydrocostuslactone in Qiwei Guangzao Wan.