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利用绿豆Berken/ACC41重组自交系在北京种植得到的121个F10家系和79个RFLP分子标记,采用改进复合区间作图法对绿豆种子休眠性和百粒重进行数量性状基因定位及上位性互作分析。检测到与发芽势有关的QTL3个,与发芽率有关的QTL4个,分别位于第1、11连锁群,解释表型变异的8.17%～12.14%和4.34%～12.69%。检测到与百粒重有关的QTL5个,分别位于第2、8、9、11连锁群,解释表型变异的4.58%～10.36%。增加发芽率和百粒重的基因效应均来自母本Berken。分别检测到发芽势、发芽率和百粒重的加性×加性上位性互作8、9、9对,对这3个性状的总表型贡献率分别达到66.58%、47.91%、39.90%。本文初步分析了休眠性和百粒重的关系,并与前人的研究结果作了比较,旨在通过分子标记辅助选择,培育适度休眠的优良绿豆品种,进而解决绿豆收获前的荚上子粒发芽问题。
121 F10 pedigrees and 79 RFLP markers were planted in Beijing by using recombinant inbred lines of mung bean Berken / ACC41, and the quantitative trait loci and epistasis of mung bean seed dormancy and 100-grain weight were studied by improved compound interval mapping do analysis. There were 3 QTLs related to germination potential and 4 QTLs related to germination rate were located in the 1st and 11th linkage groups, accounting for 8.17% ~ 12.14% and 4.34% ~ 12.69% of the phenotypic variance. Five QTLs associated with 100-kernel weight were detected, located in the 2nd, 8th, 9th and 11th linkage groups respectively, accounting for 4.58% -10.36% of phenotypic variation. Gene effects of increased germination and 100-grain weight were all from the mother, Berken. Eight, nine and nine pairs of additive × additive epistatic interactions were detected respectively, and the total phenotypic contribution rates of these three traits were 66.58%, 47.91% and 39.90%, respectively. . In this paper, the relationship between dormancy and 100-grain weight was preliminarily analyzed and compared with previous studies. The aim was to cultivate good dormant fine varieties of mung bean through molecular marker-assisted selection, problem.