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目的:研究葛根素对糖尿病大鼠肾脏氧化应激和肾功能的影响。方法:Wistar大鼠36只,随机分为正常对照组、糖尿病对照组和葛根素治疗组各12只,使用链脲佐菌素制备糖尿病大鼠模型。治疗组给予葛根素,8周后测定各组生化指标,运用比色法测定肾脏皮质丙二醛含量和肾脏组织内在抗氧化酶活性。结果:糖尿病对照组过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性均低于正常对照组,差异有统计学意义(P<0.05),皮质丙二醛水平高于正常对照组,差异有统计学意义(P<0.05)。葛根素治疗组过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶活性高于糖尿病对照组,差异有统计学意义(P<0.05),丙二醛降低,差异有统计学意义(P<0.05)。结论:葛根素能有效抑制糖尿病大鼠肾脏氧化应激反应,改善肾功能。
Objective: To study the effect of puerarin on oxidative stress and renal function in diabetic rats. Methods: Thirty-six Wistar rats were randomly divided into normal control group, diabetic control group and 12 puerarin treatment groups. Streptozotocin was used to prepare diabetic rat model. The treatment group was given puerarin. After 8 weeks, the biochemical indexes of each group were measured. The colorimetric method was used to determine the content of malondialdehyde in the kidney cortex and the intrinsic antioxidant enzyme activity in the kidney tissue. Results: The activities of catalase, superoxide dismutase, and glutathione peroxidase in the diabetic control group were lower than those in the normal control group (P<0.05). Coral malondialdehyde levels were higher than those in the normal control group. In the normal control group, the difference was statistically significant (P<0.05). Puerarin treatment group catalase, superoxide dismutase, glutathione peroxidase activity was higher than the diabetic control group, the difference was statistically significant (P <0.05), malondialdehyde decreased, the difference was statistically significant Significance (P < 0.05). Conclusion: Puerarin can effectively inhibit renal oxidative stress and improve renal function in diabetic rats.