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作者选用3种商用含铝佐剂,其中样品A、B(B的表面积大于A)各含3%的Al(OH)_3,C含2.16%AlPO_4。勃姆石的零电荷点为7.7～9.4,磷酸羟铝为4.0。两种蛋白质为溶菌酶和白蛋白,溶菌酶的分子量为14600,其等电点为11.0,白蛋白的分子量为69000,等电点为4.8,在生理pH7.35条件下,溶菌酶和勃姆石带正电荷,而白蛋白和磷酸羟铝带负电荷。在20ml(含0.85mg铝/0.5ml)的悬液中进行吸附试验。将含34mg铝的佐剂溶于等渗的NaCl溶液中,并使温度达到37℃将适量蛋白质在37℃溶于pH为7.35的正常生理盐水中,混合,用佐剂悬液混合使最终体积
The authors selected three commercially available aluminum-containing adjuvants, where Samples A and B (B has a surface area greater than A) each contained 3% Al (OH) 3 and C contained 2.16% AlPO4. The charge-free point of boehmite is 7.7 to 9.4, and the aluminum hydroxyphosphate is 4.0. The two proteins are lysozyme and albumin. The molecular weight of lysozyme is 14,600, the isoelectric point is 11.0, the molecular weight of albumin is 69,000 and the isoelectric point is 4.8. Under the condition of physiological pH7.35, lysozyme and Boehm The stone belt is positively charged, while albumin and the aluminum hydroxy phosphate are negatively charged. Adsorption experiments were performed in 20 ml (containing 0.85 mg aluminum / 0.5 ml) suspension. An adjuvant containing 34 mg of aluminum was dissolved in an isotonic NaCl solution and brought to a temperature of 37 [deg.] C. The appropriate amount of protein was dissolved in normal saline at a pH of 7.35 at 37 [deg.] C., mixed and the adjuvant suspension mixed to bring the final volume