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ＩＬ－１β对醋氨酚（ＡＡＰ）引起小鼠在体肝脏的损伤具有保护作用，本工作旨在观察ＩＬ－１β抗肝损伤效应是直接作用于肝细胞发挥的还是通过其它途径间接发挥的，探讨ＩＬ－１β抗肝损伤作用的传递机制。方法向无血清培养２４小时的原代肝细胞加人２０ｍＭｏｌ／Ｌ的ＡＡＰ，在加醋氨酚前６０ｍｉｎ和ｓｏｗｉｎ，向不同组分别加人ＩＬ－１β和／或Ｌ－ＮＡＭＥ（Ｎ－ｎｉｔｒｏ－Ｌ－ａｒｇｉｎｉｎｅｍｅｔｈｙｌｅｓｔｅｒ）。加ＡＡＰ后继续培养一段时间，测培养液中ＡＬＴ和ＡＳＴ的活性及肝细胞内ｃＡＭＰ和ｃＧＭＰ的含量。结果ＩＬ－１β可完全抑制醋氨酚引起的肝细胞培养液中ＡＬＴ、ＡＳＴ的升高，不能逆转醋氨酚引起的肝细胞内ｃＡＭＰ含量的降低，但与正常组相比，ＩＬ－１β可提高正常肝细胞内ＣＧＭＰ的含量（Ｐ＜０．０５）和醋氨酚损伤组的肝细胞内ｃＧＭＰ的含量（Ｐ＜０．０１）。加人ＮＯ合酶抑制剂Ｌ－ＮＡＭＥ可抑制ＩＬ－１肝细胞保护作用。结论ＩＬ－１β对肝细胞有直接的保护作用，其可能是一种有效的肝保护剂。这种保护可能与ＩＬ－１β引起的肝细胞内ＮＯ、ｃＧＭＰ改变有关。
IL-1β has a protective effect on the hepatic injury induced by acetaminophen (AAP) in mice. This work aims to observe whether the anti-hepatic injury effect of IL-1β acts directly on hepatocytes or indirectly through other pathways, To investigate the mechanism of IL-1β-induced liver injury. Methods Primary hepatocytes cultured in serum-free medium for 24 hours were treated with 20 mMol / L AAP, 60 min before sarcophenolate and sowin, IL-1β and / or L-NAME (N-nitro- L-arginine methylester. After adding AAP to continue training for a period of time, measure the activity of ALT and AST in culture medium and the contents of cAMP and cGMP in hepatocytes. Results IL-1β could completely inhibit the increase of ALT and AST in acetaminophen-induced hepatocyte culture medium, and could not reverse the decrease of cAMP level in acetaminophen-induced hepatocytes. Compared with the normal group, IL-1β (P <0.05) and the content of cGMP in hepatocytes of acetaminophen-induced injury group (P <0.01). Addition of NO synthase inhibitor L-NAME can inhibit IL-1 hepatocyte protective effect. Conclusion IL-1β has a direct protective effect on hepatocytes, which may be an effective hepatoprotective agent. This protection may be associated with IL-1β-induced intracellular NO, cGMP changes.