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横纹肌样瘤（ＲＴ）的起源迄今仍是世界上悬而未决的重大难题。第一例ＲＴ发现于肾脏。但迄今发现的ＲＴ均难以复制成功。在纯化３代的肿瘤阴性对照猫肾和犬肾原代细胞皮下接种裸鼠的致癌／致瘤率为０％（０／３２），肿瘤阳性对照Ｈｅｌａ细胞皮下接种裸鼠产生进行性生长恶性肿瘤的比率为１００％（４０／４０）的前提下，ＢＨＫ－２１细胞皮下接种裸鼠均产生进行性生长的恶性肿瘤（６５／６５），其中染色体众数为４０±２（比率５８％～６８％）的ＫＡ株致ＲＴ比率为５２．３８％（１１／２１），具有两个染色体众数４０±２（比率４１％～５３．５）和７２±２（比率３１％～３５％）的ＹＡ株致ＲＴ的比率为８３．３３％（１０／１２），染色体众数为７３±３（比率４２％～５０％）的成瘤细胞体外再培养ＮＭ２８／ＹＡ株致ＲＴ的比率为７１．４３％（１５／２１），染色体众数为４２（比率２８％～３０％）的Ｍ株致ＲＴ的比率为０％（０／１０），致形态类似于分化比较好的平滑肌肉瘤的恶性肿瘤的比率为１００％（１０／１０），可见ＢＨＫ－２１细胞不能用作病毒活疫苗培养基质，可替代Ｈｅｌａ细胞用作恶性肿瘤阳性对照细胞。高变异率ＢＨＫ－２１细胞株皮下接种无胸腺裸鼠大多产生恶性ＲＴ（２?
The origins of rhabdomyosarcomas (RT) are by far the major problems in the world that remain unanswered. The first case of RT was found in the kidneys. However, the RTs so far found difficult to copy successfully. The tumorigenicity / tumorigenicity rate of nude mice were 3% (0/32). The tumor-positive control Hela cells were subcutaneously inoculated with nude mice to develop malignant tumors (65/65), the chromosome number was 40 ± 2 (ratio 58% -68) in the nude mice bearing subcutaneously inoculation of BHK-21 cells (100%, 40/40) %) Of KA strains produced 52.38% (11/21) of RT, with two chromosomal patterns of 40 ± 2 (41% -53.5%) and 72 ± 2 (31% -35%) The ratio of RT induced by YA strain was 83.33% (10/12) and chromosome number 73 ± 3 (42% -50%) in vitro. 43% (15/21), and the ratio of RT to M in chromosome 42 (28% -30%) was 0% (0 10), resulting in a morphology similar to the well-differentiated leiomyosarcoma malignant tumors was 100% (10/10), it can be seen BHK-21 cells can not be used as a live virus vaccine culture medium, can replace Hela cells for malignancy as positive Control cells. High mutation rate of BHK-21 cells subcutaneously inoculated athymic nude mice mostly produce malignant RT (2?