论文部分内容阅读
目的 探讨外周血淋巴细胞携带风疹病毒抗原与风疹病毒感染中枢神经系统的关系。方法 BALB/c小鼠分别给予临床常用的可影响机体免疫功能的药物 ,再经腹腔感染风疹病毒 ,并在感染后的 1、3、7、14d观察外周血淋巴细胞携带病毒抗原的情况 ,分析其与中枢神经系统病毒感染的关系。结果 地塞米松药物组在不同时间抗原的平均携带率分别为 3 1%、4 1%、9 6 %、2 4% ,环磷酰胺药物组分别为 14 2 %、12 7%、9 9%、3 1% ,未用药物干预的感染组分别为 4 6 3 %、10 2 5 %、6 88%、1 75 %。方差分析显示 ,3组动物在感染风疹病毒后的第 2 4小时外周血淋巴细胞携带抗原存在明显差异 ,F =0 0 317,P <0 0 5。组间两两比较结果显示 :环磷酰胺药物组动物外周血淋巴细胞抗原的携带明显高于其它实验动物组 ,地塞米松药物组和药物未干预组动物之间无明显差异。确切概率法分析表明 ,动物外周血淋巴细胞风疹病毒的持续性携带与中枢神经系统风疹病毒感染的关系极为密切 ,P <0 0 0 1。结论 环磷酰胺可能影响风疹病毒感染后外周血淋巴细胞对病毒的携带率。在感染初期 ,外周血淋巴细胞持续携带风疹病毒将增大中枢神经系统感染的机会
Objective To investigate the relationship between rubella virus antigen carried by peripheral blood lymphocytes and rubella virus infection in the central nervous system. Methods BALB / c mice were given drugs commonly used in clinical practice that could affect the immune function of the mice. The rubella virus was infected intraperitoneally. The virus antigens carried by the peripheral blood lymphocytes were observed on the 1st, 3rd, 7th and 14th days after infection Its relationship with central nervous system virus infection. Results The average carrying rates of antigens in the dexamethasone group were 31%, 41%, 96% and 24%, respectively. The cyclophosphamide group was 14 2%, 12 7% and 9 9% , 3 1% respectively. The rates of infection without medication were 46.3%, 102.5%, 68.8% and 175% respectively. Variance analysis showed that there was a significant difference in the number of peripheral blood lymphocytes carrying antigens between the 3 groups at 24 hours after infection with rubella virus (F = 0 0 317, P <0 05). Comparison between groups showed that there was no significant difference in the carrier of peripheral blood lymphocyte antigens between the cyclophosphamide group and other experimental animals. There was no significant difference between the dexamethasone group and the non-intervention group. The exact probabilistic analysis showed that the persistent carrier of peripheral blood lymphocyte rubella virus in animals was closely related to CNV rubella virus infection (P <0.01). Conclusion Cyclophosphamide may affect the carrier rate of virus in peripheral blood lymphocytes after rubella virus infection. In the early stages of infection, the continued introduction of rubella virus by peripheral blood lymphocytes will increase the chance of CNS infection