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目的:建立红花黄芪配伍总黄酮制备的最佳工艺。方法:采用水提醇沉法,以紫外分光光度法测定总黄酮含量,HPLC法测定羟基红花黄色素A(HYSA)的含量,对补阳还五汤中红花黄芪配伍总黄酮类的提取工艺进行系统研究,考察提取温度、提取时间、浸提料液比和浸提次数对提取效果的影响,用AB-8大孔吸附树脂对总黄酮部位进行精制纯化。结果:最佳工艺条件为:16倍量水,60℃,提取3次,每次2 h。AB-8树脂纯化后,黄酮纯度达78.26%,羟基红花黄色素A含量为10.07%。结论:该工艺简便,合理,对红花黄芪总黄酮有效部位的分离纯化较好。
Objective: To establish the best technology for the preparation of total flavonoids of Radix Astragali and Radix Astragali. Methods: The content of total flavonoids was determined by UV-spectrophotometry with water extraction and alcohol precipitation method. The content of HYSA was determined by HPLC. The extraction process of total flavonoids from Radix Astragali and Radix Astragali in Buyang Huanwu Decoction The effects of extraction temperature, extraction time, the ratio of extract to liquid and the number of extraction on the extraction were systematically investigated. The total flavones were purified by AB-8 macroporous adsorption resin. Results: The optimum conditions were: 16 times the amount of water, 60 ℃, extraction 3 times, each 2 h. After purification of AB-8 resin, the purity of flavonoids reached 78.26% and the content of hydroxysafflor yellow A was 10.07%. Conclusion: The method is simple and reasonable, and the separation and purification of the effective fractions of total flavonoids from Carthamus tinctorius is better.