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用高度纯化的人解脲支原体免疫家兔,获得兔抗人解脲支原体抗血清,以此抗血清(一抗)及羊抗兔 IgG 荧光抗体(二抗),分别处理20例正常生育男性和20例精液解脲支原体培养阳性的不明原因不育男性的精子,并在荧光显微镜下观察。结果显示:在不育男性部分精子的头部和(或)尾部有特异性的较强荧光结合,表明有解脲支原体吸附,并有大量畸形精子,多数为卷尾畸形。此外,用比浊法检测了正常生育男性与上述不育男性精液中快速运动相精子的百分含量(FRM)及平均速度(VRM)。不育男性组比生育组的 FRM 及 VRM 都显著降低(分别为 P<0.01和 P<0.001)。本研究首次用特异性方法证实解脲支原体吸附于不育男性精子表面,提示解脲支原体感染可能通过影响精子的形态、精子的运动和精卵识别过程而导致男性不育。
The rabbits were immunized with highly purified human Ureaplasma urealyticum to obtain rabbit anti-human Ureaplasma urealyticum antiserum. The antiserum (primary antibody) and goat anti-rabbit IgG fluorescent antibody (secondary antibody) were used to treat 20 normal fertile males and 20 cases of sperm of Ureaplasma urealyticum culture positive sperm of unexplained infertile men and observed under a fluorescence microscope. The results showed that: in male infertility part of the sperm head and tail (or tail) with a strong specific fluorescence binding, indicating Ureaplasma urealyticum adsorption, and a large number of abnormal sperm, most of the coil tail deformity. In addition, the percentages (FRM) and mean velocity (VRM) of fast-moving spermatozoa in normal and male infertile men were measured by turbidimetry. The FRM and VRM were significantly lower in infertile men than in the fertility group (P <0.01 and P <0.001, respectively). This study for the first time using a specific method to confirm the adsorption of Ureaplasma urealyticum in male infertile male sperm surface, suggesting that Ureaplasma urealyticum infection may cause male infertility by affecting sperm morphology, sperm motility and sperm-egg recognition process.