论文部分内容阅读
目的建立一种能快速检测黄病毒属病毒的CODEHOP RT-PCR方法。方法根据GenBank发表的不同黄病毒多聚蛋白氨基酸序列,利用CODEHOP方法设计合成一对引物,建立能快速检测黄病毒属所有病毒的CODEHOP RT-PCR方法,并用3种不同病毒株对该方法进行特异性和灵敏度评价。结果建立的CODEHOP RT-PCR能对黄病毒RNA进行特异性扩增,目的片段的大小(400~500bp)和序列与预期结果相符。该方法对黄病毒核酸的最小检出量为8pg。结论建立的CODEHOP RT-PCR方法特异强、灵敏高,可用于黄病毒的检测。
Objective To establish a rapid RT-PCR method for CODEHOP detection of flavivirus. Methods Based on the amino acid sequences of different flavivirus polyketides published in GenBank, CODEHOP method was used to design and synthesize a pair of primers. CODEHOP RT-PCR method was established to detect all viruses of flavivirus rapidly. Three different virus strains were used to identify Sexual and Sensitive Evaluation. Results The CODEHOP RT-PCR was used to amplify the flavivirus RNA. The size of the target fragment (400 ~ 500bp) and its sequence were consistent with the expected results. The method of detection of flavivirus nucleic acid minimum 8pg. Conclusion The established CODEHOP RT-PCR method is specific and sensitive and can be used for the detection of flavivirus.