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目的研究妊娠期大鼠对卡氏肺孢子菌(Pneumocystis carinii,Pc)的易感性,为妇女孕期保健及优生优育提供参考。方法采用皮下注射地塞米松的方法建立大鼠肺孢子菌肺炎(pneumocystis pneumonia,PCP)模型,作为环境中Pc的感染来源。于实验的第6周处死2只PCP大鼠,取肺组织印片,用改良四胺银(GMS)染色,镜检Pc的感染情况。妊娠组、非妊娠组大鼠各取10只,于实验的第6周末将鼠笼置于PCP模型大鼠的鼠笼两侧,实验第7、8、9周分别取3只、3只和4只妊娠组及非妊娠组大鼠,乙醚麻醉下处死并取肺组织,提取DNA,PCR扩增Pc的mtLSUrRNA。结果PCP模型组大鼠肺印片GMS染色后油镜下观察,可见大量Pc包囊,证明PCP模型建立成功。PCR扩增妊娠组8只大鼠Pc阳性,感染率为80.00%(8/10);非妊娠组6只大鼠Pc阳性,感染率60.00%(6/10)。经Fisher确切概率法检验,两组大鼠的Pc感染率差异无统计学意义(P>0.05)。结论健康妊娠期大鼠对外源性Pc普遍易感,但感染率与非妊娠大鼠无明显差别。
Objective To study the susceptibility of pregnant rats to Pneumocystis carinii (Pc) in pregnancy and to provide references for women’s health during pregnancy and prenatal and postnatal care. Methods A rat model of pneumocystis pneumonia (PCP) was established by subcutaneous injection of dexamethasone as a source of PC infection in the environment. Two PCP rats were sacrificed on the 6th week of the experiment. The lung tissue was taken and printed with modified tetraamine silver (GMS). Pregnant group, non-pregnant group of 10 rats each at the end of the experiment the squirrel cage PCP model rats placed on both sides of the cage at the 7th, 8th and 9th weeks were taken 3, 3 and Four pregnant and non-pregnant rats were sacrificed under ether and lung tissue was taken out. DNA was extracted and the mtLSUrRNA of Pc was amplified by PCR. Results PCP model rats lung imprint GMS staining observed by oil microscopy, a large number of cysts can be seen PCP model to establish a successful. PCR amplification of pregnant rats 8 rats were positive for Pc, the infection rate was 80.00% (8/10); non-pregnant group of 6 rats Pc was positive, the infection rate was 60.00% (6/10). The Fisher exact test showed that there was no significant difference in PC infection rates between the two groups (P> 0.05). Conclusion Healthy pregnant rats are generally susceptible to exogenous Pc, but the infection rate is not significantly different from that of non-pregnant rats.