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目的观察白细胞呼吸暴发过程对白细胞形态行为改变的影响，探讨白细胞氧化暴发与相应白细胞形态行为变化特征及其相互关系。为临床白细胞流变行为和功能状态的快速诊断提供新方法。方法采集２０例体外循环（冠脉搭桥和风心换瓣病人）转流前升主动脉血样，分离白细胞。加入ＦＭＬＰ激活剂，３７℃孵化后，置于超弱化学发光仪中，计算机自动描记白细胞呼吸暴发发光计数。根据曲线过程０、１、２、３、４五个时点同步观察高放大（ １００００倍）布氏显微镜下白细胞形态、行为改变并录相，作相关分析、结果１．用ＦＭＬＰ激活白细胞呼吸暴发，化学发光计数明显增加，持续达１ｈ。随着计数曲线上升、峰值、下降变化同步观察白细胞形态行为也发生特征性改变。计数曲线分为０（上升前）、１（开始上升）、２（上升顶峰）、３（下降支）、４（终末支）共５个时点，并根据相应时点白细胞形态行为变化特征（形体、伪足、颗粒、核膜结构）分为５种细胞亚型，即Ａ型（活化前期或静止期）、Ｂ型（氧化暴发初期）、Ｃ型（暴发极期）、Ｄ型（暴发后期）、Ｅ型（失活化期或凋亡期），按白细胞氧化暴发活性归为３个时相：即未活化期表现为Ａ型细胞、暴发活化期的初期、极期、晚期分别表现为Ｂ型、Ｃ型和Ｄ型细胞；失活化
Objective To observe the effect of leukocyte respiration outbreak on morphological changes of white blood cells and to investigate the characteristics of leukocyte oxidative outbreak and the corresponding behavior of white blood cells and their correlation. Provide a new method for the rapid diagnosis of clinical leukocyte rheological behavior and functional status. Methods 20 cases of extracorporeal circulation (coronary artery bypass grafting and rheumatic heart valve replacement) were collected before ascending aorta blood flow, and white blood cells were separated. After adding FMLP activator, incubating at 37 ℃, placed in ultra-weak chemiluminescence instrument, the computer automatically tracing white cell respiratory burst luminescence count. According to the curve of five points at 0, 1, 2, 3 and 4, the leukocyte morphology under high magnification (10000 times) microscope was observed synchronously. The behavior was changed and recorded. Activation of leukocyte respiration with FMLP, chemiluminescence count significantly increased for up to 1h. With the rise of the counting curve, the changes of the peak and the descending of the leukocyte morphology simultaneously observed the characteristic changes. The counting curve is divided into five stages: 0 (before rising), 1 (starting to rise), 2 (rising peak), 3 (falling branch) and 4 (ending branch), and according to the change characteristics of the morphology of white blood cells There are five cell subtypes: A (pre-activation or quiescent), B (early onset of oxidation), C (outbreak), D-type Outbreak of late), E type (deactivation or apoptosis), according to leukocyte oxidation outbreak activity is grouped into three phases: the unactivated phase showed type A cells, the initial stage of the outbreak of activation, the very period, late, respectively The performance of B, C and D-type cells; deactivation