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采用φ(硫酸)=1%的甲醇溶液酯化法(方法1)和酸碱结合甲酯化法(方法2)分别对红树林内生真菌Paecilomyces sp.(Tree1-7)代谢产物的石油醚部位衍生化,用气相色谱-质谱法分析鉴定了其成分,测定了各成分的相对质量分数。两种方法鉴定出共有主要脂肪酸成分6种,分别为9,12-十八碳二烯酸、棕榈酸、硬脂酸、顺-9-十八碳烯酸、12-甲基-十四烷酸和Cyclopentaneundecanoic acid。其中9,12-十八碳二烯酸为红树林内生真菌Paecilomyces sp.(Tree1-7)石油醚部位的主要成分,其相对质量分数达56%以上。但两种方法所得微量脂肪酸种类存在差异,方法1共检测到13种,其中微量脂肪酸7种,而方法2能检测到17种,其中微量脂肪酸11种。与φ(硫酸)=1%的甲醇溶液酯化法相比,酸碱结合甲酯化法所得脂肪酸成分更为全面。
The petroleum ether (Paecilomyces sp. (Tree 1-7) metabolite of mangrove endophyte was separated by the esterification method of methanol (φ) (sulfuric acid) = 1% The derivatized sites were identified by gas chromatography-mass spectrometry and their relative mass fractions were determined. The two methods identified six main common fatty acid components, namely 9,12-octadecadienoic acid, palmitic acid, stearic acid, cis-9-octadecenoic acid, 12-methyl- Acid and Cyclopentaneundecanoic acid. Among them, 9,12-octadecadienoic acid is the main component of the petroleum ether part of mangrove endophytic Paecilomyces sp. (Tree1-7), and its relative mass fraction is more than 56%. However, there were differences in the types of trace fatty acids obtained by the two methods. Totally 13 species were detected in Method 1, of which 7 were trace fatty acids. However, 17 were detected by Method 2, of which 11 were trace fatty acids. Compared with the esterification of methanol solution of φ (sulfuric acid) = 1%, the fatty acid composition obtained by acid-base combined with methyl esterification is more comprehensive.