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采用连续提取法对菜芙蓉花乙醇提取物进行分离,得到石油醚相、乙酸乙酯相、正丁醇相和萃余水溶物4个不同极性部位。以还原力和清除羟自由基(·OH)、超氧阴离子自由基(O_2~-·)、DPPH自由基(DPPH·)能力为评价指标筛选其活性部位,并对活性最强部位进行体外小鼠红细胞溶血、肝组织匀浆和肝线粒体丙二醛(malondialdehyde,MDA)生成量及其肿胀度的研究。结果表明:菜芙蓉花提取物不同极性部位对自由基的清除能力表现为DPPH·>O_2~-·>·OH,其中乙酸乙酯部位(Ethyl acetate Extract,EA)对三者清除能力最强,半清除浓度EC50分别为3.52、44.96、376.87μg/m L。在体外脂质过氧化抑制作用研究中,EA能够显著抑制小鼠红细胞溶血(p<0.05),在50~500μg/m L范围内能抑制肝组织匀浆和肝线粒体MDA的生成,并有效抑制小鼠肝线粒体的肿胀,具有量效关系。
Continuous extraction method was used to separate the ethanol extracts of hibiscus flower, and petroleum ether phase, ethyl acetate phase, n-butanol phase and raffinate aqueous solution were obtained. The active site was screened for its reducing power and ability of scavenging hydroxyl radical (· OH), superoxide anion radical (DP_2 ·) and DPPH · radical (DPPH ·), and the most active sites were screened in vitro Rat erythrocyte hemolysis, liver homogenate and hepatic mitochondria malondialdehyde (MDA) production and swelling degree of the study. The results showed that the scavenging capacity of free radicals in different polarity parts of H. hibiscus L. extract was DPPH ·> O 2 ~ - · · · OH, in which Ethyl acetate Extract (EA) , Half-clearing concentration EC50 were 3.52,44.96,376.87μg / m L. EA could significantly inhibit the hemolysis of mouse erythrocytes (p <0.05) in vitro, and inhibited the formation of liver homogenate and mitochondrial MDA in the range of 50 ~ 500μg / m L Mice liver mitochondria swelling, with a dose-effect relationship.