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目的：建立用生物法和ＨＰＬＣ测定左氧氟沙星血药浓度的方法，为临床合理用药提供依据。方法：生物法使用培养基Ⅱ号和金葡球菌。ＨＰＬＣ用３００ｇ·Ｌ－１三氯醋酸沉淀蛋白，流动相为甲醇０．０２ｍｏｌ·Ｌ－１磷酸二氢钾溶液（３∶７，１ｍｏｌ·Ｌ－１磷酸调节ｐＨ＝３．０），检验波长２９０ｎｍ．结果：生物法测定不同浓度左氧氟沙星的平均回收率在９５．９０％～１０６．６８％范围内，日内和日间ＲＳＤ低于６％。ＨＰＬＣ测定不同浓度左氧氟沙星的平均回收率在９７．２４％～１０１．７０％范围内，日内和日间ＲＳＤ低于５％。６例患者以两种方式ｐｏ左氧氟沙星片剂后，用生物法和ＨＰＬＣ测其血药浓度，结果经方差分析及ｔ检验分析，无显著性差异（Ｐ＞０．０５）。结论：生物法价廉、省时、结果准确可靠；ＨＰＬＣ灵敏度高、分析速度快、分离效果好。这两种方法可以根据具体的实验条件选用
OBJECTIVE: To establish a method for the determination of levofloxacin blood concentration by biological method and HPLC, and to provide basis for clinical rational drug use. Methods: Biological method using medium No. Ⅱ and Staphylococcus aureus. HPLC 300g · L-1 trichloroacetic acid precipitation of protein, the mobile phase methanol 0.02mol · L-1 potassium dihydrogen phosphate solution (3: 7, 1mol · L-1 phosphoric acid to adjust the pH = 3.0), test Wavelength 290nm. Results: The average recoveries of different concentrations of levofloxacin in the range of 95.90% -106.68% were determined by the bioassay method. The intra-day and inter-day RSDs were less than 6%. The average recoveries of different concentrations of levofloxacin by HPLC ranged from 97.24% to 101.70%, and the intra-day and inter-day RSDs were less than 5%. Six patients in two ways po levofloxacin tablets, the biological and HPLC determination of plasma concentration, the results of analysis of variance and t test analysis, no significant difference (P> 0.05). Conclusion: Bio-method is cheap, time-saving and the result is accurate and reliable; HPLC has high sensitivity, fast analysis speed and good separation effect. These two methods can be selected according to the specific experimental conditions