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本文报道了使用气相色谱以火焰离子检测器(GC-FID)和以氮磷检测器(GC-NPD)两种方法,采用内标法,测定人尿样中异喹胍(DB)及其体内主要代谢物4-羟异喹胍(4-OH-DB)的含量。实验结果表明,应用GC-FID和GC-NPD操作简便,DB、内标物、4-OH-DB彼此分离好,灵敏度和精密度高,DB和4-OH-DB的标准曲线相关性好和回收率合格。根据上述指标,GC-NPD略优于GC-FID;75名中国正常志愿者一次口服DB 10mg采集尿样分析结果比较,GC-FID和GC-NPD两法有较好的相关系数(r=0.8712)。上述方法为研究DB羟化代谢及人群中药物羟化代谢缺陷提供了可靠的定量方法。
In this paper, we report the use of gas chromatography with flame ionization detector (GC-FID) and nitrogen and phosphorus detector (GC-NPD) two methods of internal standard method for the determination of human urine sample of quidziguanidine (DB) and its in vivo The major metabolite 4-hydroxyquinolizine (4-OH-DB) content. The experimental results show that GC-FID and GC-NPD are easy to operate, the separation of DB, internal standard and 4-OH-DB are good, the sensitivity and precision are high, and the correlation curve between DB and 4-OH-DB is good Recycling rate of qualified. According to the above indexes, GC-NPD was slightly better than GC-FID. A good correlation coefficient was obtained between GC-FID and GC-NPD (r = 0.8712 ). The above method provides a reliable quantitative method for studying the hydroxylation metabolism of DB and the hydroxylation metabolism defects in the population.