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目的观察旋毛虫对C57BL/6小鼠体内Hepa1-6肝癌细胞生长的抑制作用。方法将C57BL/6小鼠随机分成8组,每组10只。第1和5、2和6、3和7组分别感染未处理旋毛虫、60Co处理和紫外线处理旋毛虫,4和8组为不感染旋毛虫对照组,1～4组和5～8组分别于接种旋毛虫前7 d和接种后11 d接种Hepa1-6肝癌细胞。荷瘤后25 d后处死小鼠,测量肿瘤体积、重量及脾脏CD3+、CD4+、CD8+淋巴细胞数量。结果未处理旋毛虫组、60Co处理组和紫外线处理组小鼠肿瘤体积和重量均显著低于对照组(P<0.05);脾脏CD3+、CD4+百分率和CD4+/CD8+、CD4+/CD3+的比值显著高于对照组(P<0.01或P<0.05)。结论未处理的旋毛虫、经60Co和紫外线处理的旋毛虫对C57BL/6小鼠体的Hepa1-6肝癌细胞的生长均有抑制作用,未处理旋毛虫的抑瘤效果最好。
Objective To observe the inhibitory effect of Trichinella spiralis on the growth of Hepa1-6 hepatocarcinoma cells in C57BL / 6 mice. Methods C57BL / 6 mice were randomly divided into 8 groups with 10 mice in each group. Groups 1 and 5, 2 and 6, 3, and 7 were infected with untreated Trichinella spiralis, 60Co treated and UV-treated Trichinella spiralis, respectively. Groups 4 and 8 were non-infected with Trichinella spiralis, Groups 1-4 and 5-8 Hepa 1-6 hepatoma cells were inoculated 7 d before inoculation and 11 d after inoculation. Twenty-five days after the tumor-bearing mice were sacrificed and the tumor volume, weight, and the number of spleen CD3 +, CD4 +, and CD8 + lymphocytes were measured. Results The tumor volume and weight of untreated Trichinella spiralis group, 60Co-treated group and UV-treated group were significantly lower than those of the control group (P <0.05). The ratio of CD3 +, CD4 + and CD4 + / CD8 + and CD4 + / CD3 + Control group (P <0.01 or P <0.05). Conclusion Untreated Trichinella spiralis, Trichinella spiralis treated with 60Co and UV light can inhibit the growth of Hepa1-6 hepatocarcinoma cells in C57BL / 6 mice. The untreated Trichinella spiralis is the best antitumor agent.