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目的寻找一种适宜鼠疫菌生长的培养基基础液(消化液),使其在培养基上生长良好,为鼠疫检测工作打下良好基础。方法选取两种牛肉(牦牛肉和黄牛肉)用同种方法制备成赫金格尔消化液,对两种消化液进行外观检查、氨基氮含量测定、鼠疫菌活菌计数观察,寻找出适宜鼠疫菌生长的最适基础液。结果对两种牛肉按同种方法制备出的消化液进行对比观察,黄牛肉制备出的消化液其上清液不透明,氨基氮含量为400 mg/L左右,未达到鼠疫菌生长所需的氨基氮范围(500~800 mg/L或更高);牦牛肉制备出的消化液其上清液透明,氨基氮含量为820 mg/L左右,在鼠疫菌生长所需的氨基氮范围。对两种消化液制成的基础培养基进行鼠疫菌活菌计数观察,牦牛肉制备出的消化液优于黄牛肉制备的消化液,差异有统计学意义(EV76paris组t=1.876,P<0.05;141组t=0.818,P<0.05)。结论鼠疫菌生长的最适基础液为牦牛肉制备出的赫金格尔消化液。
Objective To find a medium (digestive juice) suitable for the growth of Y. pestis and to make it grow well on the culture medium, laying a good foundation for the plague detection. Methods Two kinds of beef (yak meat and yellow beef) were selected to be made into Heckinger’s digestive juice by the same method. The appearance of the two kinds of digestive juice was examined, the amino nitrogen content was determined, the viable count of Yersinia pestis was observed, and the suitable plague The most suitable base for bacterial growth. Results The digestive juice prepared by the same method was compared with the two kinds of beef. The supernatant of the digestive juice prepared by the yellow beef was opaque with amino nitrogen content of about 400 mg / L, which did not reach the amino groups needed for the growth of Y. pestis Nitrogen range (500 ~ 800 mg / L or higher); the supernatant of the yak meat prepared digestion liquid is transparent and the amino nitrogen content is about 820 mg / L, which is required for the growth of Yersinia pestis in the range of amino nitrogen. The viable counts of Yersinia pestis in the basal media made from two kinds of digestive juice were better than digestive juice prepared from yak meat. The difference was statistically significant (t = 1.876 for EV76paris group, P <0.05 ; Group 141 t = 0.818, P <0.05). Conclusion The optimum basal fluid for the growth of Yersinia pestis is Heckinger’s digestive juice prepared from yak meat.