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目的观察乳腺癌原发灶及淋巴结转移灶中血管内皮生长因子(VEGF)及转移抑制基因(m23)的表达变化及意义。方法选择2005年1~12月在我院行手术治疗且术前均未行抗癌治疗的60例乳腺癌患者,术后病理证实无同侧腋窝淋巴结转移24例(无转移组),伴有同侧腋窝淋巴结转移36例(转移组),采用免疫组化SP法检测乳腺癌原发灶和淋巴结转移灶中VEGF、nm23的表达,并分析VEGF与nm23的相关性。结果转移组原发灶中VEGF阳性表达率为91.7%,转移灶中为61.1%,无转移组VEGF阳性表达率为70.8%,转移组原发灶中VEGF阳性表达率明显高于转移灶及无转移组(P均<0.05)。转移组nm23阳性表达率在原发灶中为16.7%,转移灶中为41.7%,无转移组nm23阳性表达率为45.8%,转移组原发灶中nm23阳性表达率明显低于转移灶及无转移组(P均<0.05)。原发灶VEGF与nm23表达呈负相关(r=-0.415,P<0.05)。原发灶VEGF、nm23表达与淋巴结转移灶中VEGF、nm23表达无相关性。结论乳腺癌原发灶中VEGF表达明显高于、nm23表达明显低于淋巴结转移灶,VEGF、nm23与乳腺癌淋巴结转移密切相关,二者在乳腺癌发生和淋巴结转移中起拮抗作用。
Objective To observe the changes and significance of vascular endothelial growth factor (VEGF) and metastasis suppressor gene (m23) in primary breast cancer and lymph node metastasis. Methods Sixty-six breast cancer patients without metastasis from ipsilateral axillary lymph node metastasis after operation were enrolled in this study. Sixty-six cases of breast cancer who underwent surgical treatment in our hospital from January to December 2005 without prior anticancer therapy were enrolled in this study. 36 cases of ipsilateral axillary lymph node metastasis (metastasis group), the immunohistochemical SP method was used to detect the expression of VEGF and nm23 in primary breast cancer and lymph node metastasis, and to analyze the correlation between VEGF and nm23. Results The positive rate of VEGF expression in the primary tumor was 91.7% in the metastasis group and 61.1% in the metastatic disease group. The positive rate of VEGF expression in the metastasis group was 70.8%. The positive rate of VEGF in the metastasis group was significantly higher than that in the metastasis group Metastasis group (all P <0.05). The positive expression rate of nm23 in metastasis group was 16.7% in primary tumor and 41.7% in metastatic tumor, the positive expression rate of nm23 in non-metastatic group was 45.8%. The positive rate of nm23 in metastatic group was significantly lower than that in metastatic tumor and non-metastatic group Metastasis group (all P <0.05). The expression of VEGF in primary tumor was negatively correlated with the expression of nm23 (r = -0.415, P <0.05). The expression of VEGF and nm23 in primary tumor had no correlation with the expression of VEGF and nm23 in lymph node metastasis. Conclusions The expression of VEGF in primary breast cancer is significantly higher than that in nm23, and the expression of nm23 is significantly lower than that in lymph node metastasis. VEGF and nm23 are closely related to lymph node metastasis of breast cancer. Both of them play an antagonistic role in the occurrence of breast cancer and lymph node metastasis.