目的了解深圳地区不同人群ＴＴＶ的感染情况。方法在ＴＴＶＯＲＦ１保守区设计两对套式引物，建立了检测ＴＴＶＤＮＡ的巢式聚合酶链反应（Ｎｅｓｔｅｄ－ＰＣＲ），用该法对深圳地区９０例一般人群、８８例职业献血员、７９例静脉毒瘾者及２９例非甲庚型肝炎病人进行ＴＴＶＤＮＡ的检测。结果ＴＴＶＤＮＡ在以上４种人群中的阳性率分别为７８％，９０％，４１７％与４４８％，前者与后两者比较差异均有显著性（Ｐ＜００５）。９０例一般人群与８８例职业献血员中，１４例ＴＴＶＤＮＡ阳性者丙氨酸转氨酶（ＡＬＴ）均正常。结论深圳地区一般人群与职业献血员中ＴＴＶ携带者较常见；静脉毒瘾者是ＴＴＶ感染的高危人群；部分非甲～庚型肝炎可能与ＴＴＶ相关 Objective To understand the prevalence of TTV in different populations in Shenzhen. Methods Two pairs of nested primers were designed in TTVORF1 conservative region. A nested polymerase chain reaction (RT-PCR) was established to detect TTVDNA. Ninety cases of general population, 88 professional blood donors, 79 intravenous Addicts and 29 cases of non-hepatitis G patients TTTVNA detection. Results The positive rates of TTVDNA in the above four populations were 78%, 90%, 417% and 448%, respectively. There was significant difference between the former and the latter (P <005 ). Of the 90 general population and 88 professional blood donors, 14 cases of TTVDNA positive ALT were normal. Conclusion TTV carriers are common among general population and professional blood donors in Shenzhen area. The intravenous drug addicts are at high risk of TTV infection. Some non-A to G hepatitis patients may be related to TTV